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An exonuclease I-assisted quencher-free 2-aminopurine aptasensor based on a multipath paper-based device for ultrasensitive detection of kanamycin

文献类型：期刊论文

作者He, Xuan1; Qi, Ji3; Song, Dean2; Fu, Xiuli1
刊名MICROCHEMICAL JOURNAL
出版日期2024-08-01
卷号203页码:8
关键词Paper-based device 2-Aminopurine Exonuclease I Kanamycin Fluorescence
ISSN号0026-265X
DOI10.1016/j.microc.2024.110827
通讯作者Qi, Ji(jqi@yic.ac.cn) ; Song, Dean(songdean@caas.cn) ; Fu, Xiuli(fuxiuli@ytu.edu.cn)
英文摘要A quencher-free multipath microfluidic paper-based analytical device (mu PAD) was constructed for ultrasensitive detection of kanamycin based on exonuclease I (Exo I)-assisted signal enhancement of 2-aminopurine (2-AP). Here, Exo I, a single-stranded DNA-specific nuclease, was introduced to fully liberate 2-AP mononucleotides to greatly enhance biosensing sensitivity. 2-AP, a fluorescent adenine analogue embedded in single-stranded DNA (ssDNA), was employed as the detection signal source. The fluorescence of 2-AP is strong in the mononucleotide state, while it can have low fluorescence and even no fluorescence in ssDNA and dsDNA, respectively. The 2-AP fluorescence probe included 2-AP DNA and kanamycin aptamer. When kanamycin was present, binding occurred between kanamycin and the aptamer, leading to ssDNA, which was further digested by Exo I. In this case, free 2AP mononucleotides were liberated, indicating strong fluorescence. In addition, the captured kanamycin was released for binding with the new aptamer, which resulted in the formation of a binding-hydrolysis-release cycle with the aid of Exo I. Under optimal conditions, this mu PAD exhibited sensitive and multipath detection of kanamycin at concentrations as low as 1.26 x 10-14 M with a wide range of 10- 13-10- 7 M. Furthermore, satisfactory results were achieved for analysing spiked kanamycin in milk and honey samples. This strategy is a very promising tool for monitoring antibiotics and evaluating the safety of animal-derived foods.
WOS关键词AMINOGLYCOSIDE ANTIBIOTICS
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001248003900001
资助机构National Natural Science Foundation of China
源URL[http://ir.yic.ac.cn/handle/133337/35929]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_山东省海岸带环境工程技术研究中心
通讯作者Qi, Ji; Song, Dean; Fu, Xiuli
作者单位1.Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China
2.Chinese Acad Agr Sci, Tobacco Res Inst, Qingdao 266101, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 He, Xuan,Qi, Ji,Song, Dean,et al. An exonuclease I-assisted quencher-free 2-aminopurine aptasensor based on a multipath paper-based device for ultrasensitive detection of kanamycin[J]. MICROCHEMICAL JOURNAL,2024,203:8.
APA He, Xuan,Qi, Ji,Song, Dean,&Fu, Xiuli.(2024).An exonuclease I-assisted quencher-free 2-aminopurine aptasensor based on a multipath paper-based device for ultrasensitive detection of kanamycin.MICROCHEMICAL JOURNAL,203,8.
MLA He, Xuan,et al."An exonuclease I-assisted quencher-free 2-aminopurine aptasensor based on a multipath paper-based device for ultrasensitive detection of kanamycin".MICROCHEMICAL JOURNAL 203(2024):8.

入库方式： OAI收割

来源：烟台海岸带研究所

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