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Chronopotentiometric sensors for antimicrobial peptide-based biosensing of Staphylococcus aureus

文献类型：期刊论文

作者Zhao, Jiarong3,4; Ding, Jiawang3; Luan, Feng4; Qin, Wei1,2,3
刊名MICROCHIMICA ACTA
出版日期2024-06-01
卷号191期号:6页码:11
关键词Chronopotentiometry Ion-selective electrode Peptide Biosensing Bacteria
ISSN号0026-3672
DOI10.1007/s00604-024-06410-4
通讯作者Ding, Jiawang(jwding@yic.ac.cn)
英文摘要Charged antimicrobial peptides can be used for direct potentiometric biosensing, but have never been explored. We report here a galvanostatically-controlled potentiometric sensor for antimicrobial peptide-based biosensing. Solid-state pulsed galvanostatic sensors that showed excellent stability under continuous galvanostatic polarization were prepared by utilizing reduced graphene oxide/poly (3,4-ethylenedioxythiophene): poly (4-styrenesulfonate) (rGO/PEDOT: PSS) as a solid contact. More importantly, the chronopotentiometric sensor can be made sensitive to antimicrobial peptides with intrinsic charge on demand via a current pulse. In this study, a positively charged antimicrobial peptide that can bind to Staphylococcus aureus with high affinity and good selectivity was designed as a model. Two arginine residues with positive charges were linked to the C-terminal of the peptide sequence to increase its potentiometric responses on the electrode. The bacteria binding-induced charge or charge density change of the antimicrobial peptide enables the direct chronopotentiometric detection of the target. Under the optimized conditions, the concentration of Staphylococcus aureus can be determined in the linear range 10-1.0 x 10(5) CFU mL(-1) with a detection limit of 10 CFU mL(-1). It is anticipated that such a chronopotentiometric sensing platform is readily adaptable to detect other bacteria by choosing the peptides.
WOS关键词ION-SELECTIVE ELECTRODES ; PULSED GALVANOSTATIC CONTROL ; MEMBRANE ELECTRODES ; CONDUCTING POLYMER ; RECOGNITION ; PULSTRODES ; COMPOSITE ; GRAPHENE ; CALCIUM ; ASSAY
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001235332900004
资助机构National Natural Science Foundation of China ; Taishan Scholar Program of Shandong Province ; Special Fund for the Scholar Program of Yantai and Shandong Province ; Key Development Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences
源URL[http://ir.yic.ac.cn/handle/133337/35992]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_近岸生态与环境实验室
通讯作者Ding, Jiawang
作者单位1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
3.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China
4.Yantai Univ, Coll Chem & Chem Engn, Yantai 264005, Peoples R China
推荐引用方式
GB/T 7714		 Zhao, Jiarong,Ding, Jiawang,Luan, Feng,et al. Chronopotentiometric sensors for antimicrobial peptide-based biosensing of Staphylococcus aureus[J]. MICROCHIMICA ACTA,2024,191(6):11.
APA Zhao, Jiarong,Ding, Jiawang,Luan, Feng,&Qin, Wei.(2024).Chronopotentiometric sensors for antimicrobial peptide-based biosensing of Staphylococcus aureus.MICROCHIMICA ACTA,191(6),11.
MLA Zhao, Jiarong,et al."Chronopotentiometric sensors for antimicrobial peptide-based biosensing of Staphylococcus aureus".MICROCHIMICA ACTA 191.6(2024):11.

入库方式： OAI收割

来源：烟台海岸带研究所

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