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  4. 中科院海岸带环境过程与生态修复重点实验室
Rapid antibiotic screening based on E. coli apoptosis using a potentiometric sensor array

文献类型：期刊论文

作者Zhang, Han1,2; Mou, Junsong1,2; Ding, Jiawang2,3,4; Qin, Wei2,3,4
刊名ANALYTICA CHIMICA ACTA
出版日期2024-04-08
卷号1297页码:8
关键词Antibiotic screening Bacteria apoptosis Potentiometric detection Peptide
ISSN号0003-2670
DOI10.1016/j.aca.2024.342378
通讯作者Ding, Jiawang(jwding@yic.ac.cn)
英文摘要Phenotypic antimicrobial susceptibility testing enables reliable antibiotic screening but requires multiple strategies to identify each phenotypic change induced by different bactericidal mechanisms. Bacteria apoptosis with typical phenotypic features has never been explored for antibiotic screening. Herein, we developed an antibiotic screening method based on the measurement of antibiotic-induced phosphatidylserine (PS) exposure of apoptotic bacteria. Phosphatidylserine externalization of E. coli that can be widely used as an apoptosis marker for antibiotics with different antibacterial mechanisms was explored. A positively charged PS-binding peptide was immobilized on magnetic beads (MBs) to recognize and capture apoptotic E. coli with PS externalization. Apoptotic E. coli binding led to the charge or charge density change of MBs-peptide, resulting in a potential change on a magneto-controlled polymeric membrane potentiometric sensor. Based on the detection of apoptotic E. coli killed by antibiotics, antibiotic screening for different classes of antibiotics and silver nanoparticles was achieved within 1.5 h using a potentiometric sensor array. This approach enables sensitive, general, and timesaving antibiotic screening, and may open up a new path for antibiotic susceptibility testing.
WOS关键词ANTIBACTERIAL ACTIVITY ; CELL-DEATH ; SILVER ; RESISTANCE ; NANOPARTICLES ; MECHANISMS ; BACTERIA ; DOTS
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001193783100001
资助机构National Natural Science Foundation of China ; Taishan Scholar Program of Shandong Province ; Special Fund for the Scholar Program of Yantai and Shandong Province ; Key Development Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences
源URL[http://ir.yic.ac.cn/handle/133337/36140]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_近岸生态与环境实验室
通讯作者Ding, Jiawang
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Shandong, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Qingdao 266237, Shandong, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Han,Mou, Junsong,Ding, Jiawang,et al. Rapid antibiotic screening based on E. coli apoptosis using a potentiometric sensor array[J]. ANALYTICA CHIMICA ACTA,2024,1297:8.
APA Zhang, Han,Mou, Junsong,Ding, Jiawang,&Qin, Wei.(2024).Rapid antibiotic screening based on E. coli apoptosis using a potentiometric sensor array.ANALYTICA CHIMICA ACTA,1297,8.
MLA Zhang, Han,et al."Rapid antibiotic screening based on E. coli apoptosis using a potentiometric sensor array".ANALYTICA CHIMICA ACTA 1297(2024):8.

入库方式： OAI收割

来源：烟台海岸带研究所

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