Zebrafish gender-specific anxiety-like behavioral and physiological reactions elicited by caffeine
文献类型：期刊论文
| 作者 | Li, Yaxi2,3; Yan, Zhi1,3; Lu, Zhen3; Li, Ke3
|
| 刊名 | BEHAVIOURAL BRAIN RESEARCH
 |
| 出版日期 | 2024-08-24 |
| 卷号 | 472页码:9 |
| 关键词 | Neurostimulator Sex specificity Anxiogenic effects Behavioral phenotype Stress hormone Physiological mechanism |
| ISSN号 | 0166-4328 |
| DOI | 10.1016/j.bbr.2024.115151 |
| 通讯作者 | Li, Ke(kli@yic.ac.cn) |
| 英文摘要 | Caffeine exerts a biphasic effect on zebrafish behavior. High doses of caffeine have been associated with increased stress and anxiety, whereas low doses have been found to enhance performance on tasks requiring focus and attention. However, the sex-specific nature of these biphasic effects on behavior and physiology remains unclear. This study assessed the behavioral responses and hormone levels in male and female zebrafish after acute exposure to caffeine ranging from 0.3 to 600 mg/L. The results showed no significant difference in caffeine intake between males and females after acute exposure at each concentration. Caffeine-induced behavioral and physiological responses indicated a threshold dosage existed between 30 and 300 mg/L. Female fish displayed increased anxiety-like behavioral phenotypes, i.e., latency to upper and freezing, whereas males exhibited more erratic movement following acute exposure to a high-dose treatment. In addition, females exhibited a significant increase in whole-body cortisol levels, while males experienced a testosterone elevation at 300 mg/L of caffeine acute exposure. There was a significant decrease in the duration of erratic movements in males treated with the androgen receptor antagonist flutamide compared to the control group. The transcriptome analysis uncovered 511 and 592 up-regulated and 761 and 922 down-regulated differential expression genes in males and females, respectively, compared to the control. The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) pathway analysis revealed that caffeine has the potential to impact various pathways in zebrafish, including phototransduction and steroid hormone biosynthesis. Our findings demonstrate that testosterone and cortisol play a combined role in regulating stress responses in both behavior and physiology. Furthermore, our study highlights the significance of encompassing both male and female zebrafish as a model system. |
| WOS关键词 | SEX-DIFFERENCES ; STRESS ; RESPONSES ; TESTOSTERONE ; PERFORMANCE ; EXPRESSION ; INCREASES ; SYMPTOMS ; NOVELTY ; MODEL |
| WOS研究方向 | Behavioral Sciences ; Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:001273673400001 |
| 资助机构 | National Natural Science Foundation of China ; Yantai Science and Technology Innovation Development Program ; Seed project of Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences ; Shandong Province of China ; Yantai Municipal City's Shuangbai Plan |
| 源URL | [http://ir.yic.ac.cn/handle/133337/35650]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 海岸带生物资源高效利用研究与发展中心 |
| 通讯作者 | Li, Ke |
| 作者单位 | 1.Yantai Univ, Sch Ocean, Yantai 264005, Peoples R China 2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yaxi,Yan, Zhi,Lu, Zhen,et al. Zebrafish gender-specific anxiety-like behavioral and physiological reactions elicited by caffeine[J]. BEHAVIOURAL BRAIN RESEARCH,2024,472:9. |
| APA | Li, Yaxi,Yan, Zhi,Lu, Zhen,&Li, Ke.(2024).Zebrafish gender-specific anxiety-like behavioral and physiological reactions elicited by caffeine.BEHAVIOURAL BRAIN RESEARCH,472,9. |
| MLA | Li, Yaxi,et al."Zebrafish gender-specific anxiety-like behavioral and physiological reactions elicited by caffeine".BEHAVIOURAL BRAIN RESEARCH 472(2024):9. |
入库方式： OAI收割
来源：烟台海岸带研究所
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