High-yield magnetosome production of Magnetospirillum magneticum strain AMB-1 in flask fermentation through simplified processing and optimized iron supplementation
文献类型：期刊论文
| 作者 | Wang, Yu2,3; Liu, Zhengyi2,3 ; Li, Wenjun2,3; Cui, Hongli2,3; Huang, Yandi1; Qin, Song2,3
|
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2024-07-20 |
| 页码 | 15 |
| 关键词 | Flask fermentation High yield Magnetosome Magnetotactic bacterium AMB-1 Pathway optimization Reductase activity |
| ISSN号 | 0141-5492 |
| DOI | 10.1007/s10529-024-03507-x |
| 通讯作者 | Qin, Song(sqin@yic.ac.cn) |
| 英文摘要 | Objectives Developing a simplified flask fermentation strategy utilizing magnetotactic bacterium AMB-1 and optimized iron supplementation for high-yield magnetosome production to address the challenges associated with magnetosome acquisition. Results A reliable processing for the pure culture of AMB-1 was established using standard laboratory consumables and equipment. Subsequently, the medium and iron supplementation were optimized to enhance the yield of AMB-1 magnetosomes. The mSLM supported higher biomass accumulation in flask fermentation, reaching an OD565 of similar to 0.7. The premixed solution of ferric quinate and EDTA-Fe (at a ratio of 0.5:0.5 and a concentration of 0.4 mmol/L) stabilized Fe3+ and significantly increased the reductase activity of AMB-1. Flask fermentations with an initial volume of 15 L were then conducted employing the optimized fermentation strategy. After two rounds of iron and nutrient supplementation, the magnetosome yield reached 185.7 +/- 9.5 mg/batch (approximately 12 mg/L), representing the highest AMB-1 flask fermentation yield to our knowledge. Conclusion A flask fermentation strategy for high-yield magnetsome production was developed, eliminating the need for bioreactors and greatly simplifying the process of magnetosome acquisition. |
| WOS关键词 | MAGNETOTACTIC BACTERIA ; NANOPARTICLES ; GROWTH |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001273147500001 |
| 资助机构 | Department of Science and Technology of Shandong Province |
| 源URL | [http://ir.yic.ac.cn/handle/133337/35708]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 通讯作者 | Qin, Song |
| 作者单位 | 1.Yantai Univ, Sch Life Sci, Yantai 264003, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, 17,Chunhui Rd, Yantai 264003, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yu,Liu, Zhengyi,Li, Wenjun,et al. High-yield magnetosome production of Magnetospirillum magneticum strain AMB-1 in flask fermentation through simplified processing and optimized iron supplementation[J]. BIOTECHNOLOGY LETTERS,2024:15. |
| APA | Wang, Yu,Liu, Zhengyi,Li, Wenjun,Cui, Hongli,Huang, Yandi,&Qin, Song.(2024).High-yield magnetosome production of Magnetospirillum magneticum strain AMB-1 in flask fermentation through simplified processing and optimized iron supplementation.BIOTECHNOLOGY LETTERS,15. |
| MLA | Wang, Yu,et al."High-yield magnetosome production of Magnetospirillum magneticum strain AMB-1 in flask fermentation through simplified processing and optimized iron supplementation".BIOTECHNOLOGY LETTERS (2024):15. |
入库方式： OAI收割
来源：烟台海岸带研究所
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