中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 烟台海岸带研究所
  3. 中国科学院烟台海岸带研究所
  4. 海岸带生物学与生物资源利用重点实验室
In silico discovery and anti-tumor bioactivities validation of an algal lectin from Kappaphycus alvarezii genome

文献类型：期刊论文

作者Xu, Tongli4,7; Wang, Yin-Chu3,5,7; Ma, Jiahao1; Cui, Yulin6; Wang, Lu2
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2024-08-01
卷号275页码:9
关键词Algal lectin Lectin-carbohydrate binding Genome mining
ISSN号0141-8130
DOI10.1016/j.ijbiomac.2024.133311
通讯作者Wang, Yin-Chu(ycwang@yic.ac.cn) ; Cui, Yulin(yulincui23@bzmc.edu.cn) ; Wang, Lu(wanglu@ytu.edu.cn)
英文摘要Lectins are proteins that bind specifically and reversibly to carbohydrates, and some of them have significant anti-tumor activities. Compared to those of lectins from land plants, there are far fewer studies on algal lectins, despite of the high biodiversity of algae. However, canonical strategies based on chromatographic featureoriented screening cannot satisfy the requirement for algal lectin discovery. In this study, prospecting for novel OAAH family lectins throughout 358 genomes of red algae and cyanobacteria was conducted. Then 35 candidate lectins and 1843 of their simulated mutated forms were virtually screened based on predicted binding specificities to characteristic carbohydrates on cancer cells inferred by a deep learning model. A new lectin, named Siye, was discovered in Kappaphycus alvarezii genome and further verified on different cancer cells. Without causing agglutination of erythrocytes, Siye showed significant cytotoxicity to four human cancer cell lines (IC50 values ranging from 0.11 to 3.95 mu g/mL), including breast adenocarcinoma HCC1937, lung carcinoma A549, liver cancer HepG2 and romyelocytic leukemia HL60. And the cytotoxicity was induced through promoting apoptosis by regulating the caspase and the p53 pathway within 24 h. This study testifies the feasibility and efficiency of the genome mining guided by evolutionary theory and artificial intelligence in the discovery of algal lectins.
WOS关键词MARINE RED ALGA ; SERRA AGGLUTININ ; CANCER-CELLS ; PROTEIN ; GRIFFITHSIN ; SPECIFICITY
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种英语
WOS记录号WOS:001269359700001
资助机构National Key Research and Development Program ; Inner Mongolian Key Technology Program ; Database of Coastal Bioresources of China in National Basic Science Data Center ; High -Performance Computing Platform of Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences
源URL[http://ir.yic.ac.cn/handle/133337/35717]  
专题海岸带生物学与生物资源利用重点实验室
海岸带生物资源高效利用研究与发展中心
通讯作者Wang, Yin-Chu; Cui, Yulin; Wang, Lu
作者单位1.Hong Kong Univ Sci & Technol, Clear Water Bay, Hong Kong 999077, Peoples R China
2.Yantai Univ, Sch Pharm, Yantai 264005, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.Shandong Univ Tradit Chinese Med, Qingdao Acad Chinese Med Sci, Qingdao 266071, Peoples R China
5.Natl Basic Sci Data Ctr, Beijing 100190, Peoples R China
6.Binzhou Med Univ, Yantai 264003, Peoples R China
7.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Xu, Tongli,Wang, Yin-Chu,Ma, Jiahao,et al. In silico discovery and anti-tumor bioactivities validation of an algal lectin from Kappaphycus alvarezii genome[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,275:9.
APA Xu, Tongli,Wang, Yin-Chu,Ma, Jiahao,Cui, Yulin,&Wang, Lu.(2024).In silico discovery and anti-tumor bioactivities validation of an algal lectin from Kappaphycus alvarezii genome.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,275,9.
MLA Xu, Tongli,et al."In silico discovery and anti-tumor bioactivities validation of an algal lectin from Kappaphycus alvarezii genome".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 275(2024):9.

入库方式： OAI收割

来源：烟台海岸带研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。