Expression and Characterization of an Efficient Alginate Lyase from Psychromonas sp. SP041 through Metagenomics Analysis of Rotten Kelp
文献类型：期刊论文
| 作者 | Wang, Ping3,4; Cai, Yi1; Zhong, Hua2; Chen, Ruiting1; Yi, Yuetao3 ; Ye, Yanrui1; Li, Lili3
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| 刊名 | GENES
 |
| 出版日期 | 2024-05-01 |
| 卷号 | 15期号:5页码:18 |
| 关键词 | alginate lyase alginate oligosaccharide metagenomic analysis brown seaweed |
| DOI | 10.3390/genes15050598 |
| 通讯作者 | Ye, Yanrui(esyanruiye@scut.edu.cn) ; Li, Lili(llli@yic.ac.cn) |
| 英文摘要 | Alginate is derived from brown algae, which can be cultivated in large quantities. It can be broken down by alginate lyase into alginate oligosaccharides (AOSs), which exhibit a higher added value and better bioactivity than alginate. In this study, metagenomic technology was used to screen for genes that code for high-efficiency alginate lyases. The candidate alginate lyase gene alg169 was detected from Psychromonas sp. SP041, the most abundant species among alginate lyase bacteria on selected rotten kelps. The alginate lyase Alg169 was heterologously expressed in Escherichia coli BL21 (DE3), Ni-IDA-purified, and characterized. The optimum temperature and pH of Alg169 were 25 degrees C and 7.0, respectively. Metal ions including Mn2+, Co2+, Ca2+, Mg2+, Ni2+, and Ba2+ led to significantly increased enzyme activity. Alg169 exhibited a pronounced dependence on Na+, and upon treatment with Mn2+, its activity surged by 687.57%, resulting in the highest observed enzyme activity of 117,081 U/mg. Bioinformatic analysis predicted that Alg169 would be a double-domain lyase with a molecular weight of 65.58 kDa. It is a bifunctional enzyme with substrate specificity to polyguluronic acid (polyG) and polymannuronic acid (polyM). These results suggest that Alg169 is a promising candidate for the efficient manufacturing of AOSs from brown seaweed. |
| WOS关键词 | SODIUM ALGINATE ; DEPOLYMERIZATION ; CLASSIFICATION ; BROWN ; ACID |
| WOS研究方向 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:001233179100001 |
| 资助机构 | National Key Research and Development Program of China |
| 源URL | [http://ir.yic.ac.cn/handle/133337/35998]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 通讯作者 | Ye, Yanrui; Li, Lili |
| 作者单位 | 1.South China Univ Technol, Sch Biol & Biol Engn, Guangzhou 510006, Peoples R China 2.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 4.Shandong Univ Tradit Chinese Med, Qingdao Acad Chinese Med Sci, Qingdao 266112, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Ping,Cai, Yi,Zhong, Hua,et al. Expression and Characterization of an Efficient Alginate Lyase from Psychromonas sp. SP041 through Metagenomics Analysis of Rotten Kelp[J]. GENES,2024,15(5):18. |
| APA | Wang, Ping.,Cai, Yi.,Zhong, Hua.,Chen, Ruiting.,Yi, Yuetao.,...&Li, Lili.(2024).Expression and Characterization of an Efficient Alginate Lyase from Psychromonas sp. SP041 through Metagenomics Analysis of Rotten Kelp.GENES,15(5),18. |
| MLA | Wang, Ping,et al."Expression and Characterization of an Efficient Alginate Lyase from Psychromonas sp. SP041 through Metagenomics Analysis of Rotten Kelp".GENES 15.5(2024):18. |
入库方式： OAI收割
来源：烟台海岸带研究所
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