Molecular characterization, expression and antibacterial function of a macin, HdMac, from Haliotis discus hannai
文献类型：期刊论文
| 作者 | Jiao, Chunli1; Ruan, Jian1; Sun, Wei2; Zhang, Xinze2; Liu, Xiaobo2; Sun, Guodong4; Liu, Caili2; Sun, Chunxiao2; Tian, Xiuhui2; Yang, Dinglong3
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| 刊名 | JOURNAL OF INVERTEBRATE PATHOLOGY
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| 出版日期 | 2024-06-01 |
| 卷号 | 204页码:8 |
| 关键词 | Haliotis discus hannai Macin Antibacterial peptides Immune responses |
| ISSN号 | 0022-2011 |
| DOI | 10.1016/j.jip.2024.108113 |
| 通讯作者 | Chen, Lizhu(dayeshangbin@163.com) ; Wang, Zhongquan(441277580@qq.com) |
| 英文摘要 | Macins are a family of antimicrobial peptides, which play multiple roles in the elimination of invading pathogens. In the present study, a macin was cloned and characterized from Pacific abalone Haliotis discus hannai (Designated as HdMac). Analysis of the conserved domain suggested that HdMac was a new member of the macin family. In non-stimulated abalones, HdMac transcripts were constitutively expressed in all five tested tissues, especially in hemocytes. After Vibrio harveyi stimulation, the expression of HdMac mRNA in hemocytes was significantly up-regulated at 12 hr (P < 0.01). RNAi-mediated knockdown of HdMac transcripts affected the survival rates of abalone against V. harveyi. Moreover, recombinant protein of HdMac (rHdMac) exhibited high antibacterial activities against invading bacteria, especially for Vibrio anguillarum. In addition, rHdMac possessed binding activities towards glucan, lipopolysaccharides (LPS), and peptidoglycan (PGN), but not chitin in vitro. Membrane integrity analysis revealed that rHdMac could increase the membrane permeability of bacteria. Meanwhile, both the phagocytosis and chemotaxis ability of hemocytes could be significantly enhanced by rHdMac. Overall, the results showed that HdMac could function as a versatile molecule involved in immune responses of H. discus hannai. |
| WOS关键词 | ANTIMICROBIAL PEPTIDES ; INNATE IMMUNITY ; MECHANISMS ; CATHELICIDINS ; DEFENSINS |
| WOS研究方向 | Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:001232050100001 |
| 资助机构 | Natural Science Foundation of Shandong Province ; Shandong Key Laboratory of Marine Ecological Restoration Open Fund ; National Natural Science Foundation of China |
| 源URL | [http://ir.yic.ac.cn/handle/133337/36032]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 海岸带生物资源高效利用研究与发展中心 |
| 通讯作者 | Chen, Lizhu; Wang, Zhongquan |
| 作者单位 | 1.Yantai Ctr Food & Drug Control, Yantai 264003, Peoples R China 2.Shandong Marine Resource & Environm Res Inst, Shandong Key Lab Marine Ecol Restorat, Yantai 264006, Peoples R China 3.Chinese Acad Sci, Muping Coastal Environm Res Stn, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 4.Tianjin Xiqing Dist Agr & Rural Dev Serv Ctr, Tianjin 300380, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiao, Chunli,Ruan, Jian,Sun, Wei,et al. Molecular characterization, expression and antibacterial function of a macin, HdMac, from Haliotis discus hannai[J]. JOURNAL OF INVERTEBRATE PATHOLOGY,2024,204:8. |
| APA | Jiao, Chunli.,Ruan, Jian.,Sun, Wei.,Zhang, Xinze.,Liu, Xiaobo.,...&Wang, Zhongquan.(2024).Molecular characterization, expression and antibacterial function of a macin, HdMac, from Haliotis discus hannai.JOURNAL OF INVERTEBRATE PATHOLOGY,204,8. |
| MLA | Jiao, Chunli,et al."Molecular characterization, expression and antibacterial function of a macin, HdMac, from Haliotis discus hannai".JOURNAL OF INVERTEBRATE PATHOLOGY 204(2024):8. |
入库方式： OAI收割
来源：烟台海岸带研究所
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