Tetraspanin CD53 regulates peripheral blood leucocytes vitality and pathogen infection in turbot (Scophthalmus maximus)
文献类型：期刊论文
| 作者 | Zhang, Jian2,4; Zhou, Dandan4; Zhang, Binzhe4; Li, Xuepeng4; Jiao, Xudong1,3
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| 刊名 | FISH & SHELLFISH IMMUNOLOGY
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| 出版日期 | 2024-03-01 |
| 卷号 | 146页码:8 |
| 关键词 | Scophthalmus maximus Tetraspanin CD53 Immunoregulation Antibody ligation |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2024.109412 |
| 通讯作者 | Zhang, Jian(zhangjian@ytu.edu.cn) ; Jiao, Xudong(xdjiao@yic.ac.cn) |
| 英文摘要 | Cluster of differentiation 53 (CD53) also known as OX44 or tetraspanin 25 (TSPAN25) is a glycoprotein belonging to the tetraspanin family. Members of the tetraspanin family are characterized by four transmembrane domains, including intracellular N- and C-termini, and small and large extracellular domains. Currently, the function of CD53 in teleost is not well understood. In this study, we identified a CD53 (named SmCD53) from turbot (Scophthalmus maximus) and examined its expression and biological activity. SmCD53 contained 231 amino acid residues and was predicted to be a tetraspanin with small and large extracellular domains. SmCD53 expression was observed in different tissues, particularly in immune-related organs. Experimental infection with bacterial or viral pathogen significantly up-regulated SmCD53 expression in a time-dependent manner. Immunofluorescence microscopy analysis showed that SmCD53 was localized on the surface of PBL and was recognized by antibody against its large extracellular domain. Ligation of SmCD53 onto PBLs with antibodies suppressed the respiratory burst activity, inflammatory reaction, and enhanced cell viability. SmCD53 knockdown significantly enhanced bacterial dissemination and proliferation in turbot. Overall, these results underscore the importance of CD53 in the maintenance of the function and homeostasis of the immune system. |
| WOS关键词 | TIME RT-PCR ; TYROSINE-PHOSPHATASE ; EXPRESSION ; MOLECULE ; NEUTROPHILS ; INDUCTION ; ANTI-CD53 ; BACTERIAL ; ANTIGEN |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:001175385300001 |
| 资助机构 | Natural Science Foundation of Shandong Province ; Development Plan of Youth Innovation Team in Colleges and Universities of Shandong Province ; Yantai University Doctoral Start-up Foundation |
| 源URL | [http://ir.yic.ac.cn/handle/133337/36302]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 通讯作者 | Zhang, Jian; Jiao, Xudong |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China 2.Yantai Univ, 30 Qingquan Rd, Yantai 264005, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China 4.Yantai Univ, Sch Ocean, Yantai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jian,Zhou, Dandan,Zhang, Binzhe,et al. Tetraspanin CD53 regulates peripheral blood leucocytes vitality and pathogen infection in turbot (Scophthalmus maximus)[J]. FISH & SHELLFISH IMMUNOLOGY,2024,146:8. |
| APA | Zhang, Jian,Zhou, Dandan,Zhang, Binzhe,Li, Xuepeng,&Jiao, Xudong.(2024).Tetraspanin CD53 regulates peripheral blood leucocytes vitality and pathogen infection in turbot (Scophthalmus maximus).FISH & SHELLFISH IMMUNOLOGY,146,8. |
| MLA | Zhang, Jian,et al."Tetraspanin CD53 regulates peripheral blood leucocytes vitality and pathogen infection in turbot (Scophthalmus maximus)".FISH & SHELLFISH IMMUNOLOGY 146(2024):8. |
入库方式： OAI收割
来源：烟台海岸带研究所
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