Whole-Genome Re-sequencing and Transcriptome Reveal Candidate Genes and Pathways Associated with Hybrid Sterility in Hermaphroditic Argopecten Scallops
文献类型：期刊论文
| 作者 | Yu, Tieying1,4; Ning, Junhao4; Wang, Fukai3; Liu, Guilong2; Wang, Quanchao4; Xu, Xin2; Wang, Chunde1,3,4; Lu, Xia4
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| 刊名 | MARINE BIOTECHNOLOGY
 |
| 出版日期 | 2023-12-01 |
| 卷号 | 25期号:6页码:891-906 |
| 关键词 | Hermaphroditic Argopecten scallops Hybrid sterility Gametogenesis Meiosis and mitosis Apoptosis |
| ISSN号 | 1436-2228 |
| DOI | 10.1007/s10126-023-10247-y |
| 通讯作者 | Lu, Xia(xialu@yic.ac.cn) |
| 英文摘要 | The interspecific hybrid scallops generated from the hermaphroditic bay scallops (Argopecten irradians) and Peruvian scal-lops (Argopecten purpuratus) showed significant heterosis in growth. However, its sterility limits large-scale hybridization and hinders the development of the scallop breeding industry. Hybrid sterility is regulated by plenty of genes and involves a range of biochemical and physiological transformations. In this study, whole-genome re-sequencing and transcriptomic analysis were performed in sterile and fertile hybrid scallops. The potential genetic variations and abnormally expressed genes were detected to explore the mechanism underlying hybrid sterility in hermaphroditic Argopecten scallops. Compared with fertile hybrids, 24 differentially expressed genes (DEGs) with 246 variations were identified to be related to fertility regulation, which were mainly enriched in germarium-derived egg chamber formation, spermatogenesis, spermatid develop-ment, mismatch repair, mitotic and meiotic cell cycles, Wnt signaling pathway, MAPK signaling pathway, calcium modu-lating pathway, and notch signaling pathway. Specifically, variation and abnormal expression of these genes might inhibit the progress of mitosis and meiosis, promote cell apoptosis, and impede the genesis and maturation of gametes in sterile hybrid scallops. Eleven DEGs (XIAP, KAZN, CDC42, MEIS1, SETD1B, NOTCH2, TRPV5, M-EXO1, GGT1, SBDS, and TBCEL) were confirmed by qRT-PCR validation. Our findings may enrich the determination mechanism of hybrid sterility and provide new insights into the use of interspecific hybrids for extensive breeding. |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001126612200009 |
| 资助机构 | the National Natural Science Foundation of China |
| 源URL | [http://ir.yic.ac.cn/handle/133337/36336]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 海岸带生物资源高效利用研究与发展中心 |
| 通讯作者 | Lu, Xia |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Yantai Spring Sea AquaSeed Ltd, Yantai 264006, Peoples R China 3.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao 266109, Shandong, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Tieying,Ning, Junhao,Wang, Fukai,et al. Whole-Genome Re-sequencing and Transcriptome Reveal Candidate Genes and Pathways Associated with Hybrid Sterility in Hermaphroditic Argopecten Scallops[J]. MARINE BIOTECHNOLOGY,2023,25(6):891-906. |
| APA | Yu, Tieying.,Ning, Junhao.,Wang, Fukai.,Liu, Guilong.,Wang, Quanchao.,...&Lu, Xia.(2023).Whole-Genome Re-sequencing and Transcriptome Reveal Candidate Genes and Pathways Associated with Hybrid Sterility in Hermaphroditic Argopecten Scallops.MARINE BIOTECHNOLOGY,25(6),891-906. |
| MLA | Yu, Tieying,et al."Whole-Genome Re-sequencing and Transcriptome Reveal Candidate Genes and Pathways Associated with Hybrid Sterility in Hermaphroditic Argopecten Scallops".MARINE BIOTECHNOLOGY 25.6(2023):891-906. |
入库方式： OAI收割
来源：烟台海岸带研究所
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