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Highly Uniform Self-Assembly of Gold Nanoparticles by Butanol-Induced Dehydration and Its SERS Applications in SARS-CoV-2 Detection

文献类型：期刊论文

作者Wu, Yixuan1; Yu, Qian1; Joung, Younju1; Jeon, Chang Su2; Lee, Seunghyun3; Pyun, Sung Hyun2; Joo, Sang-Woo4; Chen, Lingxin5,6; Choo, Jaebum1
刊名ANALYTICAL CHEMISTRY
出版日期2023-08-18
页码9
ISSN号0003-2700
DOI10.1021/acs.analchem.3c01348
通讯作者Pyun, Sung Hyun(ceo@speclipse.com) ; Joo, Sang-Woo(sjoo@ssu.ac.kr) ; Chen, Lingxin(lxchen@yic.ac.cn) ; Choo, Jaebum(jbchoo@cau.ac.kr)
英文摘要Wereport the development of a reproducible and highly sensitivesurface-enhanced Raman scattering (SERS) substrate using a butanol-inducedself-assembly of gold nanoparticles (AuNPs) and its application asa rapid diagnostic platform for severe acute respiratory syndromecoronavirus 2 (SARS-CoV-2). The butanol-induced self-assembly processwas used to generate a uniform assembly of AuNPs, with multiple hotspots,to achieve high reproducibility. When an aqueous droplet containingAuNPs and target DNAs was dropped onto a butanol droplet, butanol-induceddehydration occurred, enriching the target DNAs around the AuNPs andincreasing the loading density of the DNAs on the AuNP surface. TheSERS substrate was evaluated by using Raman spectroscopy, which showedstrong electromagnetic enhancement of the Raman signals. The substratewas then tested for the detection of SARS-CoV-2 using SERS, and avery low limit of detection (LoD) of 3.1 x 10(-15) M was obtained. This provides sufficient sensitivity for the SARS-CoV-2screening assay, and the diagnostic time is significantly reducedas no thermocycling steps are required. This study demonstrates amethod for the butanol-induced self-assembly of AuNPs and its applicationas a highly sensitive and reproducible SERS substrate for the rapiddetection of SARS-CoV-2. The results suggest the potential of thisapproach for developing rapid diagnostic platforms for other biomoleculesand infectious diseases.
WOS关键词NANOSTRUCTURES ; AGGREGATION ; IMMUNOASSAY ; SUPPRESSION ; PARTICLES
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001050803100001
资助机构National Research Foundation of Korea ; National Natural Science Foundation of China
源URL[http://ir.yic.ac.cn/handle/133337/36860]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_山东省海岸带环境工程技术研究中心
通讯作者Pyun, Sung Hyun; Joo, Sang-Woo; Chen, Lingxin; Choo, Jaebum
作者单位1.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea
2.Speclipse Inc, R&D Ctr, Seongnam 13461, South Korea
3.Hanyang Univ ERICA, Dept Chem & Mol Engn, Ansan 15588, South Korea
4.Soongsil Univ, Dept Chem, Seoul 06978, South Korea
5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
6.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Wu, Yixuan,Yu, Qian,Joung, Younju,et al. Highly Uniform Self-Assembly of Gold Nanoparticles by Butanol-Induced Dehydration and Its SERS Applications in SARS-CoV-2 Detection[J]. ANALYTICAL CHEMISTRY,2023:9.
APA Wu, Yixuan.,Yu, Qian.,Joung, Younju.,Jeon, Chang Su.,Lee, Seunghyun.,...&Choo, Jaebum.(2023).Highly Uniform Self-Assembly of Gold Nanoparticles by Butanol-Induced Dehydration and Its SERS Applications in SARS-CoV-2 Detection.ANALYTICAL CHEMISTRY,9.
MLA Wu, Yixuan,et al."Highly Uniform Self-Assembly of Gold Nanoparticles by Butanol-Induced Dehydration and Its SERS Applications in SARS-CoV-2 Detection".ANALYTICAL CHEMISTRY (2023):9.

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来源：烟台海岸带研究所

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