Molecular fluorescent probes for imaging and evaluation of peroxynitrite fluctuations in living cells and in vivo under hypoxic stress
文献类型：期刊论文
| 作者 | Huang, Yan5; Yu, Lei1; Fu, Lili5; Hou, Junjun3; Wang, Lingxiao5; Sun, Mingzhao5; Wang, Xiaoyan2,5; Chen, Lingxin2,4,5
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| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2022-11-01 |
| 卷号 | 370页码:7 |
| 关键词 | Fluorescent probe Peroxynitrite Hypoxia stress Near infrared Fluorescence imaging |
| DOI | 10.1016/j.snb.2022.132410 |
| 通讯作者 | Chen, Lingxin(lxchen@yic.ac.cn) |
| 英文摘要 | Hypoxia stress causes cell damage and apoptosis by producing excessive reactive oxygen species (ROS), which is a universal concern in biology and medicine. Peroxynitrite (ONOO-) plays a vital part in the oxidative injury of tissue proteins in the pathogenic mechanism of many disorders, which is a one of ROS. Thus, excessive production of ONOO- may be one of the crucial factors leading to hypoxia stress injury. However, due to the unstable chemical property and strong reactivity of ONOO-, most of the existing detection ways cannot image and detect its level in real time. Herein, in order to selectively imaging and detecting ONOO- in living cells and in vivo, we synthesized a fluorescent probe Cy-CF3 (our fluorescent probe for detection of ONOO-). Cy-CF3 was capable of monitoring ONOO- with excellent sensitivity and selectivity. Using Cy-CF3, the relationship between hypoxia stress and ONOO- was evaluated. The probe was also employed to sense the change of ONOO- in zebrafish under hypoxia conditions as well as determine ONOO- in living mice with acute liver ischemia. Taken together, the proposed probe maybe a powerful chemical tool for the study of ONOO- in biological mechanisms. |
| WOS关键词 | REACTIVE OXYGEN ; MITOCHONDRIA ; CONSTRUCTION ; 2-PHOTON ; DISEASE ; DNA |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| 语种 | 英语 |
| WOS记录号 | WOS:000866237800003 |
| 资助机构 | National Nature Science Foundation of China ; science and technology innovation development plan of Yantai of China ; Research Initiation Fund of Binzhou Medical University ; Taishan Scholar Project Special Funding ; Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization ; Weifang Science and technology development project |
| 源URL | [http://ir.yic.ac.cn/handle/133337/37305]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 烟台海岸带研究所_山东省海岸带环境工程技术研究中心 |
| 通讯作者 | Chen, Lingxin |
| 作者单位 | 1.Weifang Univ Sci & Technol, Shandong Peninsula Engn Res Ctr Comprehens Brine, Weifang 262700, Peoples R China 2.Chinese Acad Sci, Shandong Key Lab Coastal Environm Proc, YICCAS,Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 3.Binzhou Med Univ Hosp, Dept Resp Med, Binzhou 256603, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Yan,Yu, Lei,Fu, Lili,et al. Molecular fluorescent probes for imaging and evaluation of peroxynitrite fluctuations in living cells and in vivo under hypoxic stress[J]. SENSORS AND ACTUATORS B-CHEMICAL,2022,370:7. |
| APA | Huang, Yan.,Yu, Lei.,Fu, Lili.,Hou, Junjun.,Wang, Lingxiao.,...&Chen, Lingxin.(2022).Molecular fluorescent probes for imaging and evaluation of peroxynitrite fluctuations in living cells and in vivo under hypoxic stress.SENSORS AND ACTUATORS B-CHEMICAL,370,7. |
| MLA | Huang, Yan,et al."Molecular fluorescent probes for imaging and evaluation of peroxynitrite fluctuations in living cells and in vivo under hypoxic stress".SENSORS AND ACTUATORS B-CHEMICAL 370(2022):7. |
入库方式： OAI收割
来源：烟台海岸带研究所
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