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Single-cell RNA sequencing revealed the roles of macromolecule epidermal growth factor receptor (EGFR) in the hybrid sterility of hermaphroditic Argopecten scallops

文献类型：期刊论文

作者Yu, Tieying1,4; Wang, Chunde1,2; Fan, Jiawei1,4; Chen, Rongjie3; Liu, Guilong5; Xu, Xin5; Ning, Junhao1; Lu, Xia6
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2024-11-01
卷号280页码:13
关键词Epidermal growth factor receptor Hybrid sterility Argopecten scallops ATP ROS
ISSN号0141-8130
DOI10.1016/j.ijbiomac.2024.136062
通讯作者Ning, Junhao(jhning@yic.ac.cn) ; Lu, Xia(luxia_0202@163.com)
英文摘要The macromolecule epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein that belongs to the protein kinase superfamily, which plays versatile functions in cell proliferation, development and fertility regulation. Almost all F1 hybrids obtained from the hermaphroditic bay scallops and Peruvian scallops exhibit infertility, and the genetic mechanism remains unclear. In this study, the comprehensive scRNA-seq was first conducted in the gonads of hybrid scallops, deducing the developmental sequence of germ cells and identifying the critical regulators in hybrid sterility: epidermal growth factor receptor. During the development from oogenesis phase germ cells to oocytes, the expression of the EGFR gene gradually decreased in sterile hybrids but increased in fertile hybrids. The significantly lower EGFR expression and ATP content, but higher ROS production rate was detected in the gonad of sterile hybrids than that in fertile hybrids, which might cause slow development of oocytes, stagnation of cell cycle, insufficient energy supply, high level of apoptosis and final sterility. Specific knock-down of EGFR gene led to decreased ATP content, increased ROS production rate, and inhibited oocyte maturation and gonadal development. These findings provide new insights into the roles of EGFR in hybrid infertility of bivalves and the healthy development of scallop breeding.
WOS关键词BAY SCALLOP ; ACTIVATION ; DIFFERENTIATION ; EXPRESSION ; EGGSHELL ; EMBRYOS ; GENE
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种英语
WOS记录号WOS:001329498600001
资助机构National Natural Science Foundation of China ; Scientific and Technological Project of Yantai, Shandong Province ; Earmarked Fund for Shandong Modern Agro-Industry Technology Research System
源URL[http://ir.yic.ac.cn/handle/133337/37135]  
专题海岸带生物学与生物资源利用重点实验室
海岸带生物资源高效利用研究与发展中心
通讯作者Ning, Junhao; Lu, Xia
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresour, Yantai 264003, Shandong, Peoples R China
2.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao 266109, Shandong, Peoples R China
3.Laizhou Marine Dev & Fishery Serv Ctr, Laizhou 261400, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Yantai Spring Sea AquaSeed Ltd, Yantai 264006, Peoples R China
6.Yantai Univ, Sch Ocean, Yantai 264005, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Yu, Tieying,Wang, Chunde,Fan, Jiawei,et al. Single-cell RNA sequencing revealed the roles of macromolecule epidermal growth factor receptor (EGFR) in the hybrid sterility of hermaphroditic Argopecten scallops[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,280:13.
APA Yu, Tieying.,Wang, Chunde.,Fan, Jiawei.,Chen, Rongjie.,Liu, Guilong.,...&Lu, Xia.(2024).Single-cell RNA sequencing revealed the roles of macromolecule epidermal growth factor receptor (EGFR) in the hybrid sterility of hermaphroditic Argopecten scallops.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,280,13.
MLA Yu, Tieying,et al."Single-cell RNA sequencing revealed the roles of macromolecule epidermal growth factor receptor (EGFR) in the hybrid sterility of hermaphroditic Argopecten scallops".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 280(2024):13.

入库方式： OAI收割

来源：烟台海岸带研究所

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