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Nonylphenol displays immunotoxicity by triggering hemocyte extracellular traps in Manila clam via ROS burst, ERK pathway and glycolysis

文献类型：期刊论文

作者Lv, Xiaojing1,2,4; Han, Yijing5; Li, Yongxue1,2; Wang, Xin1,2; Zhang, Tianyu1,2; Wang, Xiaodan1,2; Zhang, Qianqian1,2; Yang, Dinglong1,2,3; Zhao, Jianmin1,2,3
刊名ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
出版日期2024-10-15
卷号285页码:14
关键词Nonylphenol Extracellular traps ERK signaling pathway Reactive oxygen species Glycolysis
ISSN号0147-6513
DOI10.1016/j.ecoenv.2024.117145
通讯作者Yang, Dinglong(dlyang@yic.ac.cn) ; Zhao, Jianmin(jmzhao@yic.ac.cn)
英文摘要Nonylphenol (NP), an endocrine disruptor, has been demonstrated to be a harmful environmental contaminant and toxic to organisms. In this study, to address concerns regarding the immunotoxicity of NP, we treated clam Ruditapes philippinarum hemocytes with NP in vitro and explored the underlying mechanisms of NP-induced extracellular traps (ETs). NP could induce the formation of hemocytes ETs in a dose-dependent manner. Transcriptomics analysis revealed changes of signaling pathway involved in immunity and energy metabolism in hemocytes after NP stimulation. In this process, both reactive oxygen species (ROS) and myeloperoxidase (MPO) were up-regulated. Moreover, mitogen-activated protein kinase (MAPK) signaling pathway was proved to be activated in the formation of NP-induced ETs, manifested as enhanced phosphorylation of extracellular signal-regulated kinase (ERK) but not p38 or c-Jun N-terminal kinase (JNK). In the presence of U0126, an ERK phosphorylation inhibitor, the NP-induced expression of NADPH oxidase enzyme (NOX) was significantly decreased, which further alleviated the ROS production and ultimately limited the release of ETs. NP exposure increased glucose uptake, along with enhanced activities of glycolysis-related enzymes such as hexokinase (HK) and pyruvate kinase (PK). After inhibiting glycolysis by the inhibitor 2-DG, the formation of NP-induced ETs was significantly suppressed. ERK could regulate mTOR signaling and the PI3K/AKT pathway, potentially directing ETs formation by orchestrating the glycolysis through the activation of key transcription factors c-Myc and HIF-1 alpha. Collectively, the results preliminary confirm that the ERK-NOX-ROS axis and glycolysis are involved in NP-induced ETs formation, contributing to the cellular immunotoxicity in clam.
WOS关键词OYSTER CRASSOSTREA-GIGAS ; PARTICULATE MATTER ; MARINE ORGANISMS ; BISPHENOL-A ; 4-NONYLPHENOL ; RELEASE ; IMMUNITY ; EXPOSURE ; FATE ; IMMUNOMODULATION
WOS研究方向Environmental Sciences & Ecology ; Toxicology
语种英语
WOS记录号WOS:001330943500001
资助机构Shandong Key Laboratory of Marine Ecological Restoration ; National Science & Technology Fundamental Resources Investigation Program of China ; Seed Project of Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences
源URL[http://ir.yic.ac.cn/handle/133337/37143]  
专题海岸带生物学与生物资源利用重点实验室
海岸带生物资源高效利用研究与发展中心
通讯作者Yang, Dinglong; Zhao, Jianmin
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresour, Yantai 264003, Peoples R China
3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Ludong Univ, Sch Fisheries, Yantai 264025, Peoples R China
推荐引用方式
GB/T 7714		 Lv, Xiaojing,Han, Yijing,Li, Yongxue,et al. Nonylphenol displays immunotoxicity by triggering hemocyte extracellular traps in Manila clam via ROS burst, ERK pathway and glycolysis[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2024,285:14.
APA Lv, Xiaojing.,Han, Yijing.,Li, Yongxue.,Wang, Xin.,Zhang, Tianyu.,...&Zhao, Jianmin.(2024).Nonylphenol displays immunotoxicity by triggering hemocyte extracellular traps in Manila clam via ROS burst, ERK pathway and glycolysis.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,285,14.
MLA Lv, Xiaojing,et al."Nonylphenol displays immunotoxicity by triggering hemocyte extracellular traps in Manila clam via ROS burst, ERK pathway and glycolysis".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 285(2024):14.

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来源：烟台海岸带研究所

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