Integrated physiological, biochemical, and transcriptomic analyses of Bruguiera gymnorhiza leaves under long-term copper stress: Stomatal size, wax crystals and composition
文献类型：期刊论文
| 作者 | Shang, Chenjing1,2; Zhou, Qiao1; Nkoh, Jackson Nkoh1,3,4; Liu, Jing1; Wang, Junjie1; Hu, Zhangli1; Hussain, Quaid1,3 |
| 刊名 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
 |
| 出版日期 | 2024-08-01 |
| 卷号 | 281页码:16 |
| 关键词 | Mangroves Stomata Photosynthetic pathway Leaf wax structure Heavy metal pollution Transcriptomic analysis |
| ISSN号 | 0147-6513 |
| DOI | 10.1016/j.ecoenv.2024.116609 |
| 英文摘要 | Copper (Cu) is a necessary mineral nutrient for plant growth and development and is involved in several morphological, physiological, and biochemical processes; however, high concentrations of Cu can negatively impact these processes. The role of stomata in responding to various biotic and abiotic stimuli has not been studied in Bruguiera gymnorhiza, particularly in terms of their coordinated interactions at the molecular, physiological, and biochemical levels. Moreover, numerous plants employ strategies such as the presence of thick waxy cuticles on their leaf epidermis and the closing of stomata to reduce water loss. Thus, this study investigates the accumulation of Cu in B. gymnorhiza and its effect on leaf morphology and the molecular response under different Cu treatments (0, 200, and 400 mg L-1, Cu0, Cu200, and Cu400, respectively) during a two years stress period. The results show that Cu stress affected accumulation and transport, increased the activities of peroxidase and ascorbate peroxidase, concentrations of soluble sugar, proline, and H2O2, and decreased the activity of catalase and content of malondialdehyde. Also, Cu-induced stress decreased the uptake of phosphorus and nitrogen and inhibited plant photosynthesis, which consequently led to reduced plant growth. Scanning electron microscopy combined with gas chromatography-mass spectrometry showed that B. gymnorhiza leaves had higher wax crystals and compositions under increased Cu stress, which forced the leaf's stomata to be closed. Also, the contents of alkanes, alcohols, primary alcohol levels (C26:0, C28:0, C30:0, and C32:0), n-Alkanes (C29 and C30), and other wax loads were significantly higher, while fatty acid (C12, C16, and C18) was lower in Cu200 and Cu400 compared to Cu0. Furthermore, the transcriptomic analyses revealed 1240 (771 up- and 469 downregulated), 1000 (723 up- and 277 down-regulated), and 1476 (808 up- and 668 downregulated) differentially expressed genes in Cu0 vs Cu200, Cu0 vs Cu400, and Cu200 vs Cu400, respectively. RNA-seq analyses showed that Cu mainly affected eight pathways, including photosynthesis, cutin, suberin, and wax biosynthesis. This study provides a reference for understanding mangrove response to heavy metal stress and developing novel management practices. |
| WOS关键词 | ANTIOXIDANT DEFENSE SYSTEM ; CUTICULAR WAXES ; COMPLEX NETWORK ; DROUGHT STRESS ; ABIOTIC STRESS ; HEAVY-METALS ; SALT STRESS ; GROWTH ; ACCUMULATION ; TOXICITY |
| 资助项目 | National Key R&D Program of China[2021YFC3101304] ; National Natural Science Foundation of China[42376219] ; National Natural Science Foundation of China[41706137] ; Hainan International Scientific and Techno-logical Cooperation R D project[GHYF2024013] |
| WOS研究方向 | Environmental Sciences & Ecology ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:001333746100001 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 资助机构 | National Key R&D Program of China ; National Natural Science Foundation of China ; Hainan International Scientific and Techno-logical Cooperation R D project |
| 源URL | [http://ir.idsse.ac.cn/handle/183446/11713]  |
| 专题 | 深海科学研究部 |
| 通讯作者 | Zhou, Qiao; Hussain, Quaid |
| 作者单位 | 1.Shenzhen Univ, Guangdong Engn Res Ctr Marine Algal Biotechnol, Shenzhen Publ Serv Platform Collaborat Innovat Mar, Coll Life Sci & Oceanog,Shenzhen Engn Lab Marine A, Shenzhen 518060, Peoples R China 2.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China 3.Shenzhen Univ, Coll Phys & Optoelect Engn, Shenzhen 518060, Peoples R China 4.Univ Buea, Dept Chem, POB 63, Buea, Cameroon |
| 推荐引用方式 GB/T 7714 | Shang, Chenjing,Zhou, Qiao,Nkoh, Jackson Nkoh,et al. Integrated physiological, biochemical, and transcriptomic analyses of Bruguiera gymnorhiza leaves under long-term copper stress: Stomatal size, wax crystals and composition[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2024,281:16. |
| APA | Shang, Chenjing.,Zhou, Qiao.,Nkoh, Jackson Nkoh.,Liu, Jing.,Wang, Junjie.,...&Hussain, Quaid.(2024).Integrated physiological, biochemical, and transcriptomic analyses of Bruguiera gymnorhiza leaves under long-term copper stress: Stomatal size, wax crystals and composition.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,281,16. |
| MLA | Shang, Chenjing,et al."Integrated physiological, biochemical, and transcriptomic analyses of Bruguiera gymnorhiza leaves under long-term copper stress: Stomatal size, wax crystals and composition".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 281(2024):16. |
入库方式： OAI收割
来源：深海科学与工程研究所
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