中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Identification of Single Yeast Budding Using Impedance Cytometry with a Narrow Electrode Span

文献类型:期刊论文

作者Liu, Xun1; Tang, Tao1; Yi, Powei2; Yuan, Yapeng3,4; Lei, Cheng5; Li, Ming6; Tanaka, Yo3,4; Hosokawa, Yoichiroh1; Yalikun, Yaxiaer1,4
刊名Sensors
出版日期2022-10
卷号22页码:7743
通讯作者Tang, Tao ; Yalikun, Yaxiaer
目次
英文摘要

Impedance cytometry is wildly used in single-cell detection, and its sensitivity is essential for determining the status of single cells. In this work, we focus on the effect of electrode gap on detection sensitivity. Through comparing the electrode span of 1 µm and 5 µm, our work shows that narrowing the electrode span could greatly improve detection sensitivity. The mechanism underlying the sensitivity improvement was analyzed via numerical simulation. The small electrode gap (1 µm) allows the electric field to concentrate near the detection area, resulting in a high sensitivity for tiny particles. This finding is also verified with the mixture suspension of 1 µm and 3 µm polystyrene beads. As a result, the electrodes with 1 µm gap can detect more 1 µm beads in the suspension than electrodes with 5 µm gap. Additionally, for single yeast cells analysis, it is found that impedance cytometry with 1 µm electrodes gap can easily distinguish budding yeast cells, which cannot be realized by the impedance cytometry with 5 µm electrodes gap. All experimental results support that narrowing the electrode gap is necessary for tiny particle detection, which is an important step in the development of submicron and nanoscale impedance cytometry.

语种英语
版本出版稿
源URL[http://ir.idsse.ac.cn/handle/183446/11660]  
专题深海工程技术部_深海资源开发研究室
通讯作者Tang, Tao; Yalikun, Yaxiaer
作者单位1.Division of Materials Science, Nara Institute of Science and Technology
2.Department of Applied Chemistry, National Yang Ming Chiao Tung University
3.Graduate School of Frontier Biosciences, Osaka University
4.Center for Biosystems Dynamics Research (BDR), RIKEN
5.The Institute of Technological Sciences, Wuhan University
6.School of Engineering, Macquarie University
推荐引用方式
GB/T 7714
Liu, Xun,Tang, Tao,Yi, Powei,et al. Identification of Single Yeast Budding Using Impedance Cytometry with a Narrow Electrode Span[J]. Sensors,2022,22:7743.
APA Liu, Xun.,Tang, Tao.,Yi, Powei.,Yuan, Yapeng.,Lei, Cheng.,...&Yalikun, Yaxiaer.(2022).Identification of Single Yeast Budding Using Impedance Cytometry with a Narrow Electrode Span.Sensors,22,7743.
MLA Liu, Xun,et al."Identification of Single Yeast Budding Using Impedance Cytometry with a Narrow Electrode Span".Sensors 22(2022):7743.

入库方式: OAI收割

来源:深海科学与工程研究所

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