Exploratory research on the effective chemical basis of tanreqing injection for treating acute lung injury: In vivo, in vitro and in silico
文献类型：期刊论文
| 作者 | Tang, Bixi5,6; Xie, Like5; Wang, Yangyang5; Shi, Yulong3; Kan, Weijuan5; Feng, Bo5; Lin, Chenxuan4,5; Xu, Zhijian3 ; Zhu, Weiliang3; Li, Jia2,4,5,6
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| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
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| 出版日期 | 2025-01-30 |
| 卷号 | 337页码:11 |
| 关键词 | Lung first-pass effect Tanreqing injection Pharmacokinetics Sepsis-associated acute lung injury Acute respiratory distress syndrome |
| ISSN号 | 0378-8741 |
| DOI | 10.1016/j.jep.2024.118780 |
| 通讯作者 | Li, Jia() ; Zhang, Xuemei() ; Tian, Xiaoting() ; Zang, Yi(yzang@lglab.ac.cn) |
| 英文摘要 | Ethnopharmacological relevance: Sepsis-induced acute lung injury (ALI) presents with significant morbidity and mortality in clinical settings. Tanreqing Injection (TRQI) has been clinically recommended for the treatment of ALI; however, the specific active chemical constituents remain unidentified. Aim of the study: This study aimed to elucidate the potential pharmacologically active components and the underlying mechanisms of TRQI in the treatment of sepsis-induced ALI. Materials and methods: High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) techniques were employed to identify the effective chemical constituents of TRQI. Additionally, an in vitro study was conducted using Raw264.7 macrophage cells stimulated with lipopolysaccharide (LPS) to evaluate the inhibitory effects of TRQI. An acute lung injury model produced by LPS was intraperitoneal injection in mice to assess the ALI-inhibitory effect of TRQI. The lung's pathological characteristics were examined using hematoxylin and eosin staining. Enzyme-linked immunosorbent assay (ELISA) and QPCR were performed to confirm the pharmaceutical effect. Network pharmacology was employed for mechanistic exploration, incorporating GO, and PPI analyses of targets. Src inhibitor and JNK agonist used to investigate the dependence of associated signaling pathways. Results: Combining pharmacokinetic characteristics, lung first-pass effect and anti-inflammatory effects, the main components of TRQI for treating sepsis induced ALI were narrowed down to seven compounds: chlorogenic acid, scutellarin, wogonoside, oroxyloside, oroxylin A and baicalein. Network pharmacology indicated that Src/JNK signaling pathway, may be the main regulatory pathway for treatment of actue lung injury. Next by using Src inhibitor, Src inhibition partly diminished the protective effects of TRQI in LPS-injected mice. Pretreatment with JNK agonist anisomycin abolished the protective effects of lung injury in vivo. Conclusions: TRQI is injected, the seven compounds could be presented in vivo, which can improve ALI by inhibiting Src-JNK signaling. |
| WOS关键词 | TRADITIONAL CHINESE MEDICINE ; SEPSIS |
| 资助项目 | National Natural Science Founda-tion of China[82151219] ; National Natural Science Founda-tion of China[21937002] ; National Natural Science Founda-tion of China[82130099] ; National Natural Science Founda-tion of China[81973456] ; Shanghai Science and Technology Development Funds[22ZR1415200] ; Shanghai Municipal Science and Technology Major Project |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:001332301300001 |
| 出版者 | ELSEVIER IRELAND LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/313793]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li, Jia; Zhang, Xuemei; Tian, Xiaoting; Zang, Yi |
| 作者单位 | 1.Lingang Lab, Shanghai 201203, Peoples R China 2.Bohai Rim Adv Res Inst Drug Discovery, Shandong Lab Yantai Drug Discovery, Yantai 264117, Shandong, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Drug Discovery & Design Ctr, State Key Lab Drug Res, Shanghai 201203, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, Stake Key Lab Chem Biol, Shanghai 201203, Peoples R China 6.Fudan Univ, Sch Pharm, Dept Pharmacol, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang, Bixi,Xie, Like,Wang, Yangyang,et al. Exploratory research on the effective chemical basis of tanreqing injection for treating acute lung injury: In vivo, in vitro and in silico[J]. JOURNAL OF ETHNOPHARMACOLOGY,2025,337:11. |
| APA | Tang, Bixi.,Xie, Like.,Wang, Yangyang.,Shi, Yulong.,Kan, Weijuan.,...&Zang, Yi.(2025).Exploratory research on the effective chemical basis of tanreqing injection for treating acute lung injury: In vivo, in vitro and in silico.JOURNAL OF ETHNOPHARMACOLOGY,337,11. |
| MLA | Tang, Bixi,et al."Exploratory research on the effective chemical basis of tanreqing injection for treating acute lung injury: In vivo, in vitro and in silico".JOURNAL OF ETHNOPHARMACOLOGY 337(2025):11. |
入库方式： OAI收割
来源：上海药物研究所
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