Rapid isolation of extracellular vesicles using covalent organic frameworks combined with microfluidic technique
文献类型：期刊论文
| 作者 | Xin, Fangyuan3,6; Ren, Xiaohong4,5; Lin, Xueyuan3,6; Ma, Wuzhen2,5; Ran, Bo6; Teng, Yupu3,6; Gao, Ping3; Wang, Caifen5; Wu, Li1,5; Cun, Dongmei6 |
| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2024-08-01 |
| 卷号 | 245页码:11 |
| 关键词 | Microfluidic Covalent organic framework Extracellular vesicle Isolation |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2024.116153 |
| 通讯作者 | Cun, Dongmei(cundongmei@163.com) ; Zhang, Jiwen(jwzhang@simm.ac.cn) |
| 英文摘要 | Extracellular vesicles (EVs) are nano-sized lipid-membrane vesicles involved in intercellular communication and reflecting the physiological and pathological processes of their parental cells. Rapid isolation of EVs with low cost is an essential precondition for downstream function exploration and clinical applications. In this work, we designed a novel EVs isolation device based on the boronated organic framework (BOF) coated recyclable microfluidic chip (named EVs-BD) to separate EVs from cell culture media. Using a reactive oxygen species responsive phenylboronic ester compound, the highly porous BOF with a pore size in the range of 10-300 nm was prepared by crosslinking gamma-cyclodextrin metal-organic frameworks. A mussel-inspired polydopamine (PDA)/ polyethyleneimine (PEI) coating was employed to pattern BOF on the PDMS substrate of microfluidic channels. The EVs-BD was demonstrated to offer distinct advantages over the traditional ultracentrifugation method, such as operation simplicity and safety, reduced time and expense, and low expertize requirements. All things considered, a novel approach of EV acquisition has been successfully developed, which can be customized easily to meet the requirements of various EV-relevant research. |
| 资助项目 | National Science and Technology Major Projects for National Key R & D Program of China[2020YFE0201700] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:001331651800001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/313799]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Cun, Dongmei; Zhang, Jiwen |
| 作者单位 | 1.Natl Inst Food & Drug Control, NMPA Key Lab Qual Res & Evaluat Pharmaceut Excipie, Beijing 100050, Peoples R China 2.Univ Chinese Acad Sci, Sch Pharm, Beijing 100049, Peoples R China 3.Yangtze Delta Drug Adv Res Inst, Nantong 226126, Peoples R China 4.Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Macau 999078, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Delivery Syst, Shanghai 201210, Peoples R China 6.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xin, Fangyuan,Ren, Xiaohong,Lin, Xueyuan,et al. Rapid isolation of extracellular vesicles using covalent organic frameworks combined with microfluidic technique[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2024,245:11. |
| APA | Xin, Fangyuan.,Ren, Xiaohong.,Lin, Xueyuan.,Ma, Wuzhen.,Ran, Bo.,...&Zhang, Jiwen.(2024).Rapid isolation of extracellular vesicles using covalent organic frameworks combined with microfluidic technique.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,245,11. |
| MLA | Xin, Fangyuan,et al."Rapid isolation of extracellular vesicles using covalent organic frameworks combined with microfluidic technique".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 245(2024):11. |
入库方式： OAI收割
来源：上海药物研究所
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