Evolutionary and molecular basis of ADP-ribosylation reversal by zinc-dependent macrodomains
文献类型：期刊论文
| 作者 | Ariza, Antonio6,7; Liu, Qiang3,4,5; Cowieson, Nathan P.2; Ahel, Ivan6; Filippov, Dmitri V.5; Rack, Johannes Gregor Matthias1 |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2024-10-01 |
| 卷号 | 300期号:10页码:20 |
| DOI | 10.1016/j.jbc.2024.107770 |
| 通讯作者 | Ahel, Ivan(ivan.ahel@path.ox.ac.uk) ; Filippov, Dmitri V.(lippov@lic.leidenuniv.nl) ; Rack, Johannes Gregor Matthias(j.rack@exeter.ac.uk) |
| 英文摘要 | Dynamic ADP-ribosylation signaling is a crucial pathway that controls fundamental cellular processes, in particular, the response to cellular stresses such as DNA damage, reactive oxygen species, and infection. In some pathogenic microbes, the response to oxidative stress is controlled by a SirTM/zinccontaining macrodomain (Zn-Macro) pair responsible for establishment and removal of the modification, respectively. Targeting this defence mechanism against the host's innate immune response may lead to novel approaches to support the fight against emerging antimicrobial resistance. Earlier studies suggested that Zn-Macros play a key role in the activation of this defence. Therefore, we used phylogenetic, biochemical, and structural approaches to elucidate the functional properties of these enzymes. Using the substrate mimetic asparagineADP-ribose as well as the ADP-ribose product, we characterize the catalytic role of the zinc ion in the removal of the ADPribosyl modification. Furthermore, we determined structural properties that contribute to substrate selectivity within the different Zn-Macro branches. Together, our data not only give new insights into the Zn-Macro family but also highlight their distinct features that may be exploited for the development of future therapies. |
| WOS关键词 | MULTIPLE SEQUENCE ALIGNMENT ; GLYCINE CLEAVAGE SYSTEM ; ANGLE SCATTERING DATA ; REACTION-MECHANISM ; PROTEIN ; IDENTIFICATION ; COORDINATION ; EXPRESSION ; SOFTWARE ; DOMAINS |
| 资助项目 | MRC Centre for Medical Mycology at the University of Exeter[MR/N006364/2] ; MRC Centre for Medical Mycology at the University of Exeter[MR/V033417/1] ; NIHR Exeter Biomedical Research Centre ; China Scholarship Council (CSC) ; Wellcome Trust[210634] ; Wellcome Trust[223107] ; Biotechnology and Biological Sciences Research Council[BB/R007195/1] ; Biotechnology and Biological Sciences Research Council[BB/W016613/1] ; Cancer Research United Kingdom[C35050/A22284] ; Medical Research Council[MR/X007472/1] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001331651300001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/313802]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Ahel, Ivan; Filippov, Dmitri V.; Rack, Johannes Gregor Matthias |
| 作者单位 | 1.Univ Exeter, Med Res Council Ctr Med Mycol, Exeter, England 2.Harwell Sci & Innovat Campus, Diamond Light Source, Didcot, Oxon, England 3.Chinese Acad Sci, Shanghai Inst Mat Med, Beijing, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Zhongshan Inst Drug Discovery, Beijing, Peoples R China 5.Leiden Univ, Leiden Inst Chem, Bioorgan Synth, Leiden, Netherlands 6.Univ Oxford, Sir William Dunn Sch Pathol, Oxford, England 7.Univ Sheffield, Sch Biosci, Sheffield, England |
| 推荐引用方式 GB/T 7714 | Ariza, Antonio,Liu, Qiang,Cowieson, Nathan P.,et al. Evolutionary and molecular basis of ADP-ribosylation reversal by zinc-dependent macrodomains[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2024,300(10):20. |
| APA | Ariza, Antonio,Liu, Qiang,Cowieson, Nathan P.,Ahel, Ivan,Filippov, Dmitri V.,&Rack, Johannes Gregor Matthias.(2024).Evolutionary and molecular basis of ADP-ribosylation reversal by zinc-dependent macrodomains.JOURNAL OF BIOLOGICAL CHEMISTRY,300(10),20. |
| MLA | Ariza, Antonio,et al."Evolutionary and molecular basis of ADP-ribosylation reversal by zinc-dependent macrodomains".JOURNAL OF BIOLOGICAL CHEMISTRY 300.10(2024):20. |
入库方式： OAI收割
来源：上海药物研究所
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