An economically viable stable isotope-enhanced multiple reaction monitoring method for total fatty acid analysis in a mouse model of non-alcoholic fatty liver disease
文献类型：期刊论文
| 作者 | Zhang, Zijia4,5; Liu, Yawen4,5; Li, Gaohan3; Chen, Xiaoling2,5; Lei, Min4,5 ; Zhou, Yang4,5; Long, Huali4,5; Chen, Qinhua1; Hou, Jinjun4,5; Wu, Wanying2,3,4,5
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| 刊名 | JOURNAL OF CHROMATOGRAPHY A
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| 出版日期 | 2024-11-08 |
| 卷号 | 1736页码:10 |
| 关键词 | Stable isotope Fatty acid Multiple reaction monitoring N-dimethylethylenediamine NAFLD |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2024.465406 |
| 通讯作者 | Hou, Jinjun(jinjun_hou@simm.ac.cn) ; Wu, Wanying(wanyingwu@simm.ac.cn) |
| 英文摘要 | The complex pathological mechanisms of non-alcoholic fatty liver disease (NAFLD) are closely related to dysregulated lipid metabolism, and the therapeutic effects of the traditional Chinese medicine Zexie-Baizhu Decoction (AA) on NAFLD have been gaining increasing attention. However, research into altered lipid metabolism, especially fatty acids, in NAFLD and the intervention of AA faces technical challenges, especially in the precise quantitative analysis of fatty acids in biological samples. The high complexity of biological matrices, particularly after drug intervention, greatly increases the difficulty of detection. Therefore, this study innovatively developed a simple and economical stable isotope derivatization technique by synthesizing d(6)N,N-dimethylethylenediamine (d(6)-DMED) in the laboratory, establishing a simple and economical method for fatty acid quantification. This method employs a chemical reaction under low-temperature conditions to ensure the efficient synthesis of d(6)-DMED. Using ultra-high performance liquid chromatography-triple quadrupole mass spectrometry technique (UHPLC-MS/MS), combined with optimized chromatographic separation conditions and dynamic multiple reaction monitoring mode, the study established a highly sensitive detection method for 35 fatty acid derivatives. Methodological evaluation showed that the limits of quantification ranged from 0.002 to 0.060 mu M, with high linearity of R-2 > 0.995. Additionally, the relative recovery rates were between 93.14% and 106.63%. To further demonstrate the feasibility of this method for fatty acid quantification, it was applied to measure fatty acids in multiple tissues in a mouse NAFLD model, as well as the effects of AA intervention on fatty acid metabolism. This rapid, simple, and cost-effective detection method not only enhances the understanding of NAFLD mechanisms but also provides a new strategy for evaluating the biological complex system after drug intervention. |
| WOS关键词 | DERIVATIZATION STRATEGY ; LIPOGENESIS |
| 资助项目 | National Natural Science Foundation of China[82374029] ; National Key Research and Development Program of China[2023YFC3504205] ; SIMM-SHUTCM Traditional Chinese Medicine Innovation Joint Research Program[2022] ; SIMM-SHUTCM Traditional Chinese Medicine Innovation Joint Research Program[E2G808H096] ; National Key R & D Program Strategic Scientific and Technological Innovation Cooperation Key Project[2022YFE0203600] ; Sanming Project of Medicine in Shenzhen[ZZYSM202106004] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001334070900001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/313817]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Hou, Jinjun; Wu, Wanying |
| 作者单位 | 1.Shenzhen Baoan Authent TCM Therapy Hosp, Shenzhen 518101, Guangdong, Peoples R China 2.Shanghai Univ Tradit Chinese Med, Sch Pharm, Shanghai 201203, Peoples R China 3.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210029, Jiangsu, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Res Ctr TCM Standardizat Technol, 501 Haike Rd, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Zijia,Liu, Yawen,Li, Gaohan,et al. An economically viable stable isotope-enhanced multiple reaction monitoring method for total fatty acid analysis in a mouse model of non-alcoholic fatty liver disease[J]. JOURNAL OF CHROMATOGRAPHY A,2024,1736:10. |
| APA | Zhang, Zijia.,Liu, Yawen.,Li, Gaohan.,Chen, Xiaoling.,Lei, Min.,...&Wu, Wanying.(2024).An economically viable stable isotope-enhanced multiple reaction monitoring method for total fatty acid analysis in a mouse model of non-alcoholic fatty liver disease.JOURNAL OF CHROMATOGRAPHY A,1736,10. |
| MLA | Zhang, Zijia,et al."An economically viable stable isotope-enhanced multiple reaction monitoring method for total fatty acid analysis in a mouse model of non-alcoholic fatty liver disease".JOURNAL OF CHROMATOGRAPHY A 1736(2024):10. |
入库方式： OAI收割
来源：上海药物研究所
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