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Online Comprehensive Two-Dimensional Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolic Profiling and Comparison Enabling the Characterization of 1146 Ginsenosides and More Explicit Differentiation of Ginseng

文献类型：期刊论文

作者Wang, Simiao2,3; Zou, Yadan2,3; Zhang, Min2,3; Xu, Xiaoyan2,3; Wang, Hongda2,3; Jiang, Meiting2,3; Hu, Ying3; Cheng, Huizhen2,3; Li, Xue2,3; Guo, Dean1,2,3
刊名JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
出版日期2024-10-23
卷号72期号:44页码:24866-24878
关键词Online comprehensive 2D-LC/QTOF-MS Untargeted metabolomics Ginseng Ginsenoside Multicomponent characterization
ISSN号0021-8561
DOI10.1021/acs.jafc.4c06793
通讯作者Wang, Hongda(hdwang_1996@163.com) ; Guo, Dean(daguo@simm.ac.cn) ; Yang, Wenzhi(wzyang0504@tjutcm.edu.cn)
英文摘要This work was designed for the in-depth characterization and holistic comparison of up to 12 ginseng varieties, which can benefit the development of functional foods and ensure their authenticity in the food industry. An online comprehensive two-dimensional liquid chromatography/quadrupole time-of-flight mass spectrometry (2D-LC/QTOF-MS) approach was established by configurating the XCharge C18 and HSS Cyano columns. Under the optimal conditions, we characterized a total of 1146 ginsenosides (including 876 potentially new compounds) from 12 ginseng varieties by reference to an in-house library of 573 known ginsenosides and 70 reference compounds. The online 2D-LC/QTOF-MS-based untargeted metabolomics workflows were developed, by which 126 potential ginsenoside markers were unveiled and utilized to establish the key identification points for each ginseng species. Compared with the conventional liquid chromatography/mass spectrometry metabolomics, our multidimensional chromatography approach performed better in discriminating multiple ginseng varieties. This work demonstrates a potent and practical methodology to identify easily confused functional plants.
WOS关键词NATURAL COMPOUNDS ; ORTHOGONALITY ; MS/MS
资助项目National Natural Science Foundation of China[82374030] ; National Natural Science Foundation of China[2023T030TJ] ; China Postdoctoral Science Foundation-Tianjin Joint Support Program[23ZYJDSS00030] ; Science and Technology Program of Tianjin in China[22HHZYSS00012] ; Science & Technology Program of Haihe Laboratory of Modern Chinese Medicine[CBQM2022103] ; Science & Technology Program of Haihe Laboratory of Modern Chinese Medicine[CBCM2023202] ; Foundation of State Key Laboratory of Component-based Chinese Medicine[23JCJQJC00030] ; Tianjin Outstanding Youth Fund
WOS研究方向Agriculture ; Chemistry ; Food Science & Technology
语种英语
WOS记录号WOS:001340183000001
出版者AMER CHEMICAL SOC
源URL[http://119.78.100.183/handle/2S10ELR8/314032]  
专题中国科学院上海药物研究所
通讯作者Wang, Hongda; Guo, Dean; Yang, Wenzhi
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China
2.Haihe Lab Modern Chinese Med, Tianjin 301617, Peoples R China
3.Tianjin Univ Tradit Chinese Med, State Key Lab Chinese Med Modernizat, Tianjin 301617, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Simiao,Zou, Yadan,Zhang, Min,et al. Online Comprehensive Two-Dimensional Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolic Profiling and Comparison Enabling the Characterization of 1146 Ginsenosides and More Explicit Differentiation of Ginseng[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2024,72(44):24866-24878.
APA Wang, Simiao.,Zou, Yadan.,Zhang, Min.,Xu, Xiaoyan.,Wang, Hongda.,...&Yang, Wenzhi.(2024).Online Comprehensive Two-Dimensional Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolic Profiling and Comparison Enabling the Characterization of 1146 Ginsenosides and More Explicit Differentiation of Ginseng.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,72(44),24866-24878.
MLA Wang, Simiao,et al."Online Comprehensive Two-Dimensional Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolic Profiling and Comparison Enabling the Characterization of 1146 Ginsenosides and More Explicit Differentiation of Ginseng".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72.44(2024):24866-24878.

入库方式： OAI收割

来源：上海药物研究所

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