Rotating culture regulates the formation of HepaRG-derived liver organoids via YAP translocation
文献类型：期刊论文
| 作者 | Zhong SY(钟绍宇); Zheng L(郑璐); Wu Y(武亿); Sun SJ(孙树津) ; Luo, Qing; Song, Guanbin; Lv DY(吕东媛); Long M(龙勉)
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| 刊名 | BMC BIOLOGY
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| 出版日期 | 2024-11 |
| 卷号 | 22期号:1页码:262 |
| 关键词 | Liver organoids HepaRG cells Rotating Flat Chamber Rotating culture YAP Mechanotransduction |
| DOI | 10.1186/s12915-024-02062-1 |
| 英文摘要 | BackgroundLiver organoid serves as an alternative model for liver pathophysiology in carbohydrate or lipid metabolism and xenobiotic metabolism transformation. Biomechanical cues including spaceflight mission can affect liver organoid construction and their related functions, but their underlying mechanisms are not fully understood yet. Here, a rotating cell culture device, namely Rotating Flat Chamber (RFC), was specifically designed for adhering cells or cell aggregated to elucidate the effects of altered gravity vector on HepaRG-derived liver organoids construction.ResultsThe organoids so formed under RFC presented the fast growth rate and large projection area. Meanwhile, the expressions of two pluripotency markers of SOX9 and CD44 were enhanced. This finding was positively correlated with the increased YAP expression and nuclear translocation as well as the elevated alpha 4 beta 6-integrin expression. Inhibition of YAP expression and nuclear translocation decreased the expression of SOX9 and CD44 under RFC, thereby attenuating the pluripotency of HepaRG-derived liver organoids.ConclusionsIn conclusion, we proposed a novel liver organoid construction method using rotating culture, by which the pluripotency of liver organoids so constructed is mediated by alpha 4 beta 6-integrin and YAP translocation. This work furthered the understanding in how the gravity vector orientation affects the construction of liver organoids and the related mechanotransductive pathways. |
| 分类号 | 二类/Q1 |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:001355199600002 |
| 资助机构 | National Key Research and Development Program of China {2021YFA0719302] ; National Natural Science Foundation of China {32130061, 32371376] ; Scientific Instrument Developing Project of the Chinese Academy of Sciences {GJJSTU20220002] ; China Manned Space Flight Technology Project Chinese Space Station Experiment Project {YYWT0901EXP0701] |
| 其他责任者 | Lue DY ; Long M |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/97160]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 作者单位 | 1.【Zhong, Shaoyu & Luo, Qing & Song, Guanbin】 Chongqing Univ, Coll Bioengn, Key Lab Biorheol Sci & Technol, Minist Educ, Chongqing 400044, Peoples R China 2.【Zheng, Lu & Wu, Yi & Sun, Shujin & Lue, Dongyuan & Long, Mian】 Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China 3.【Zhong, Shaoyu & Zheng, Lu & Wu, Yi & Sun, Shujin & Lue, Dongyuan & Long, Mian】 Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China 4.【Zhong, Shaoyu & Zheng, Lu & Wu, Yi & Sun, Shujin & Lue, Dongyuan & Long, Mian】 Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing Key Lab Engn Construction & Mechanobiol, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhong SY,Zheng L,Wu Y,et al. Rotating culture regulates the formation of HepaRG-derived liver organoids via YAP translocation[J]. BMC BIOLOGY,2024,22(1):262. |
| APA | 钟绍宇.,郑璐.,武亿.,孙树津.,Luo, Qing.,...&龙勉.(2024).Rotating culture regulates the formation of HepaRG-derived liver organoids via YAP translocation.BMC BIOLOGY,22(1),262. |
| MLA | 钟绍宇,et al."Rotating culture regulates the formation of HepaRG-derived liver organoids via YAP translocation".BMC BIOLOGY 22.1(2024):262. |
入库方式： OAI收割
来源：力学研究所
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