Neutrophils exhibit flexible migration strategies and trail formation mechanisms on varying adhesive substrates
文献类型：期刊论文
| 作者 | Gao, Wenbo; Zhang XN(张晓宁) ; Hu WH(胡文慧); Han J(韩杰); Liu, Xiaoheng; Zhang Y(章燕); Long M(龙勉)
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| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2025-03 |
| 卷号 | 314页码:122881 |
| 关键词 | Neutrophil migration Trail release ICAM-1 PLL Migration strategy |
| ISSN号 | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2024.122881 |
| 英文摘要 | Substrate anchorage is essential for cell migration, and actin polymerization at cell front and myosin contractility at cell rear are known to govern cell forward movement. Yet their differential driving strategies for neutrophil migration on distinct adhesiveness substrates and their contributions to the migration-induced trail formation remain unclear. Here we explore the morphological changes, migration dynamics, and trail formation of neutrophils on ICAM-1 and PLL substrates, with a focus on the relationships among adhesive forces, traction forces, and out-of-plane forces. Results indicate that, on ICAM-1, neutrophil migration and trail formation rely on the coordinated interactions of Arp2/3 and myosin, along with biochemical regulation ( via Syk and calpain) of adhesion and de-adhesion. This pattern leads to traction forces being concentrated at relatively fewer adhesive sites, facilitating cell forward migration. On PLL, however, neutrophils primarily depend on Arp2/3-mediated actin polymerization, resulting in a broader distribution of traction forces and weaker adhesions, which allows for higher leading-edge migrating velocities. Elevated membrane tension and out-of-plane forces generated by bleb protrusions on PLL reduce the reliance on myosin-driven contraction at the trailing edge, enabling easier tail detachment through elastic recoil. This work highlights the differential impact of substrate adhesiveness on neutrophil migration and trail formation and dynamics, providing new insights into cell migration mechanisms and potential therapeutic targets for inflammatory and immune-related disorders. |
| 分类号 | 一类 |
| WOS研究方向 | Engineering ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001343979000001 |
| 资助机构 | National Natural Science Foundation of China {32250017, T2394514, 32130061, 12272389] ; Scientific Instrument Developing Project of the Chinese Academy of Sciences {GJJSTU20220002] |
| 其他责任者 | Zhang Y ; Long M ; Liu XH |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/97191]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 作者单位 | 1.【Gao, Wenbo & Liu, Xiaoheng】 Sichuan Univ, Inst Biomed Engn, West China Sch Basic Med Sci & Forens Med, Chengdu 610041, Sichuan, Peoples R China 2.【Zhang, Xiaoning & Han, Jie & Zhang, Yan & Long, Mian】 Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China 3.【Gao, Wenbo & Zhang, Xiaoning & Hu, Wenhui & Han, Jie & Zhang, Yan & Long, Mian】 Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China 4.【Gao, Wenbo & Zhang, Xiaoning & Hu, Wenhui & Han, Jie & Zhang, Yan & Long, Mian】 Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Wenbo,Zhang XN,Hu WH,et al. Neutrophils exhibit flexible migration strategies and trail formation mechanisms on varying adhesive substrates[J]. BIOMATERIALS,2025,314:122881. |
| APA | Gao, Wenbo.,张晓宁.,胡文慧.,韩杰.,Liu, Xiaoheng.,...&龙勉.(2025).Neutrophils exhibit flexible migration strategies and trail formation mechanisms on varying adhesive substrates.BIOMATERIALS,314,122881. |
| MLA | Gao, Wenbo,et al."Neutrophils exhibit flexible migration strategies and trail formation mechanisms on varying adhesive substrates".BIOMATERIALS 314(2025):122881. |
入库方式： OAI收割
来源：力学研究所
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