中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 力学研究所
  3. 中国科学院力学研究所
  4. 国家微重力实验室
Stretch-induced hepatic endothelial mechanocrine promotes hepatocyte proliferation

文献类型：期刊论文

作者Wu Y(武亿); Li LD(李林达); Li, Wang; Li N(李宁); Zhang XY(张晓宇); Zheng L(郑璐); Zhong SY(钟绍宇); Lv SQ(吕守芹); Shu XY(舒芯钰); Zhou J(周瑾)
刊名HEPATOLOGY
出版日期2024-09
关键词HB-EGF liver regeneration liver sinusoidal endothelial cell mechanotransduction mechanical stretch
ISSN号0270-9139
DOI10.1097/HEP.0000000000001082
英文摘要Background and Aims:Partial hepatectomy-induced liver regeneration causes the increase in relative blood flow rate within the liver, which dilates hepatic sinusoids and applies mechanical stretch on liver sinusoidal endothelial cells (LSECs). Heparin-binding EGF-like growth factor is a crucial growth factor during liver regeneration. We aimed to investigate whether this sinusoidal dilation-induced stretch promotes HB-EGF secretion in LSECs and what the related molecular mechanism is. Approach and Results: In vivo partial hepatectomy, ex vivo liver perfusion, and in vitro LSEC mechanical stretch were applied to detect HB-EGF expression in LSECs and hepatocyte proliferation. Knockdown or inhibition of mechanosensitive proteins was used to unravel the molecular mechanism in response to stretch. This stretch triggers amplitude-dependent and duration-dependent HB-EGF upregulation in LSECs, which is mediated by Yes-associated protein (YAP) nuclear translocation and binding to TEA domain family. This YAP translocation is achieved in 2 ways: On one hand, F-actin polymerization-mediated expansion of nuclear pores promotes YAP entry into nucleus passively. On the other hand, F-actin polymerization upregulates the expression of BAG family molecular chaperone regulator 3, which binds with YAP to enter the nucleus cooperatively. In this process, beta 1-integrin serves as a target mechanosensory in stretch-induced signaling pathways. This HB-EGF secretion-promoted liver regeneration after 2/3 partial hepatectomy is attenuated in endothelial cell-specific Yap1-deficient mice. Conclusions:Our findings indicate that mechanical stretch-induced HB-EGF upregulation in LSECs through YAP translocation can promote hepatocyte proliferation during liver regeneration through a mechanocrine manner, which deepens the understanding of the mechanical-biological coupling in liver regeneration.
分类号一类
WOS研究方向Gastroenterology & Hepatology
语种英语
WOS记录号WOS:001329010700001
资助机构National Key Research and Development Program of China {2021YFA0719302] ; National Natural Science Foundation of China {32130061, T2394514, 32371376] ; Scientific Instrument Developing Project of the Chinese Academy of Sciences {GJJSTU20220002]
其他责任者Lü DY ; Long M
源URL[http://dspace.imech.ac.cn/handle/311007/97194]  
专题力学研究所_国家微重力实验室
作者单位1.【Gao, Ming & Liu, Sijin】 Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing, Peoples R China
2.【Ai, Ding】 Tianjin Med Univ, Dept Physiol & Pathophysiol, Tianjin, Peoples R China
3.【Wu, Yi & Li, Wang & Li, Ning & Zhang, Xiaoyu & Zheng, Lu & Lue, Shouqin & Shu, Xinyu & Zhou, Jin & Gao, Ming & Liu, Sijin & Lue, Dongyuan & Long, Mian】 Univ Chinese Acad Sci, Sch Engn Sci, Beijing, Peoples R China
4.【Wu, Yi & Li, Linda & Li, Wang & Li, Ning & Zhang, Xiaoyu & Zheng, Lu & Zhong, Shaoyu & Lue, Shouqin & Shu, Xinyu & Zhou, Jin & Lue, Dongyuan & Long, Mian】 Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Natl Micrograv Lab, 15 Beisihuanxi Rd, Beijing 100190, Peoples R China
5.【Wu, Yi & Li, Linda & Li, Wang & Li, Ning & Zhang, Xiaoyu & Zheng, Lu & Zhong, Shaoyu & Lue, Shouqin & Shu, Xinyu & Zhou, Jin & Lue, Dongyuan & Long, Mian】 Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing Key Lab Engn Construct & Mechanobiol, 15 Beisihuanxi Rd, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714		 Wu Y,Li LD,Li, Wang,et al. Stretch-induced hepatic endothelial mechanocrine promotes hepatocyte proliferation[J]. HEPATOLOGY,2024.
APA 武亿.,李林达.,Li, Wang.,李宁.,张晓宇.,...&龙勉.(2024).Stretch-induced hepatic endothelial mechanocrine promotes hepatocyte proliferation.HEPATOLOGY.
MLA 武亿,et al."Stretch-induced hepatic endothelial mechanocrine promotes hepatocyte proliferation".HEPATOLOGY (2024).

入库方式： OAI收割

来源：力学研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。