Isolation of highly polar galloyl glucoside tautomers from Saxifraga tangutica through preparative chromatography and assessment of their in vitro antioxidant activity
文献类型：期刊论文
| 作者 | Tong, Yingying; Chu, Ming; Zhou, Jia; Wang, Qilan ; Li, Gang; Abd El-Aty, A. M.; Dang, Jun
|
| 刊名 | BMC CHEMISTRY
 |
| 出版日期 | 2024 |
| 卷号 | 18期号:1 |
| 英文摘要 | In this work, the rapid and efficient preparation of isolated galloyl glucoside tautomer free radical inhibitors was investigated using Saxifraga tangutica as a raw material. Four highly polar galloyl glucoside tautomers, 3-O-galloyl-alpha-d-glucose reversible arrow 3-O-galloyl-beta-d-glucose (Fr2-1-1), 2-O-galloyl-alpha-d-glucose reversible arrow 2-O-galloyl-beta-d-glucose (Fr2-1-2/2-1-3), 1-O-galloyl-beta-d-glucose (Fr2-2-1), and 6-O-galloyl-alpha-d-glucose reversible arrow 6-O-galloyl-beta-d-glucose (Fr2-3-1/Fr2-3-2), were obtained via two-step medium-pressure liquid chromatography (with solid loading instead of conventional liquid injection) and one-step high-performance chromatography coupled with on-line RPLC-DPPH techniques for targeted isolation. This separation integration technique not only increases sample intake and reduces time cost but also visualizes each step of targeted separation. All four compounds were isolated from the plant for the first time. In vitro antioxidant activity assays by DPPH (1,1-diphenyl-2-picrylhydrazyl) revealed that Fr2-1-2/Fr2-1-3 (IC50: 5.52 +/- 0.32 mu M), Fr2-2-1 (IC50: 7.22 +/- 0.57 mu M), and Fr2-3-1/Fr2-3-2 (IC50: 7.36 +/- 0.25 mu M) had superior free radical scavenging abilities and that both were superior to that of quercetin (IC50: 18.61 +/- 3.55 mu M). Oxidative stress assays revealed that Fr2-1-2/Fr2-1-3 significantly inhibited oxidative stress damage in H2O2-induced HepG2 cells, decreased the level of ROS (P < 0.01) and protected hepatocytes. Combined with the current results, gallic acid showed greater antioxidant activity when H atoms were replaced at d-glucose -OH (C-2) than at the other three sites [-OH (C-1), -OH (C-6) and -OH (C-3)]. |
| 源URL | [http://210.75.249.4/handle/363003/61909]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Tong, Yingying,Chu, Ming,Zhou, Jia,et al. Isolation of highly polar galloyl glucoside tautomers from Saxifraga tangutica through preparative chromatography and assessment of their in vitro antioxidant activity[J]. BMC CHEMISTRY,2024,18(1). |
| APA | Tong, Yingying.,Chu, Ming.,Zhou, Jia.,Wang, Qilan.,Li, Gang.,...&Dang, Jun.(2024).Isolation of highly polar galloyl glucoside tautomers from Saxifraga tangutica through preparative chromatography and assessment of their in vitro antioxidant activity.BMC CHEMISTRY,18(1). |
| MLA | Tong, Yingying,et al."Isolation of highly polar galloyl glucoside tautomers from Saxifraga tangutica through preparative chromatography and assessment of their in vitro antioxidant activity".BMC CHEMISTRY 18.1(2024). |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。