BSC2 modulates AmB resistance via the maintenance of intracellular sodium/potassium ion homeostasis in Saccharomyces cerevisiae
文献类型：期刊论文
| 作者 | Huang, Zhiwei; Xiao, Fulong; Wang, Qiao; Zhang, Xiaojuan; Shen, Yuhu ; Deng, Yunxia; Shi, Ping
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| 刊名 | RESEARCH IN MICROBIOLOGY
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| 出版日期 | 2024 |
| 卷号 | 175期号:8 |
| 英文摘要 | Previous studies on BSC2 have shown that it enhances yeast cell resistance to AmB via antioxidation and induces multidrug resistance by contributing to biofilm formation. Herein, we found that BSC2 over- expression could reverse the sensitivity of pmp30 to AmB and help the tested strains restore the intracellular sodium/potassium balance under exposure to AmB. Meanwhile, overexpression of the chitin gene CHS2 could simulate BSC2 to reverse the sensitivity of pmp30 and nha10 to high salt or AmB. However, BSC2 overexpression in flo110 failed to induce AmB resistance, form biofilms, and affect cell wall biogenesis, while CHS2 overexpression compensated the resistance of flo110 to AmB. Additionally, BSC2 levels were positively correlated with maintaining cell membrane integrity under exposure to AmB, CAS, or a combination of both. BSC2 overexpression in nha10 exhibited a similar function of CHS2, which can compensate for the sensitivity of the mutant to high salt. Altogether, the results demonstrate for the first time that BSC2 may promote ion equilibrium by strengthening cell walls and inhibiting membrane damage in a FLO path-dependent manner, thus enhancing the resistance of yeast cells to AmB. This study also reveals the possible mechanism of antifungal drugs CAS and AmB combined to inhibit fungi. (c) 2024 Institut Pasteur. Published by Elsevier Masson SAS. All rights are reserved, including those for text and data mining, AI training, and similar technologies. |
| 源URL | [http://210.75.249.4/handle/363003/61914]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Huang, Zhiwei,Xiao, Fulong,Wang, Qiao,et al. BSC2 modulates AmB resistance via the maintenance of intracellular sodium/potassium ion homeostasis in Saccharomyces cerevisiae[J]. RESEARCH IN MICROBIOLOGY,2024,175(8). |
| APA | Huang, Zhiwei.,Xiao, Fulong.,Wang, Qiao.,Zhang, Xiaojuan.,Shen, Yuhu.,...&Shi, Ping.(2024).BSC2 modulates AmB resistance via the maintenance of intracellular sodium/potassium ion homeostasis in Saccharomyces cerevisiae.RESEARCH IN MICROBIOLOGY,175(8). |
| MLA | Huang, Zhiwei,et al."BSC2 modulates AmB resistance via the maintenance of intracellular sodium/potassium ion homeostasis in Saccharomyces cerevisiae".RESEARCH IN MICROBIOLOGY 175.8(2024). |
入库方式： OAI收割
来源：西北高原生物研究所
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