Exploring the effect and mechanism of Hippophae rhamnoides L. triterpenoid acids on improving NAFLD based on network pharmacology and experimental validation in vivo and in vitro
文献类型：期刊论文
| 作者 | Ren, Lichengcheng; Wang, Ruinan; Wang, Yue; Tie, Fangfang; Dong, Qi; Wang, Honglun ; Hu, Na
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| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
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| 出版日期 | 2024 |
| 卷号 | 335 |
| 英文摘要 | Ethnopharmacological relevance: Sea buckthorn ( Hippophae rhamnoides L.) is a traditional Chinese medicinal and possesses a rich medical history in terms of treating gastric disorders, sputum and cough and liver injuries in oriental medicinal system. By reason of the complicated chemical constituents, the material basis and potential pharmacological mechanism of sea buckthorn acting on Non-alcoholic fatty liver disease (NAFLD) has not been clearly elucidated. Aim of the study: To explore the pharmacological efficacy and underlying mechanism of sea buckthorn triterpenoid acid enrichment (STE) in the treatment of NAFLD. Materials and methods: The approaches of Network pharmacology and experiment validation in vitro and in vivo were applied in this study. Firstly, targets of triterpenoid acid compounds and NAFLD were collected from databases. The crucial targets were screened by the construction of protein-protein interaction (PPI) network. Furthermore, the potential signaling pathways and targets affected by STE was predicted by GO together with KEGG enrichment analysis. Finally, the experiment validation was carried out through high-fat feeding NAFLD mice and lipid accumulation HepG2 cell model. Lipids and liver related biochemical indicators were determined, Oil Red O and H&E &E staining were employed to observe fat accumulation. In addition, the expression levels of proteins of key target and signal pathway anticipated in network pharmacology were detected to elaborated its action mechanism. Results: A total of 180 intersecting potential targets for enhancing NAFLD with STE were eventually identified. 6 key targets including AKT1, TNF, IL6, INS, JUN, STAT3 and TP53 were further identified and the AMPK-SREBP1 pathway was enriched. Animal experiment result showed that STE treatment could significantly reduce the levels of TG, TC, LDL-C, ALT and AST, increase the levels of HDL-C in serum, and improve lipid accumulation of epididymal fat and liver. The results of the lipid accumulation cell model indicated that STE and key compound oleanolic acid could diminish intracellular lipid levels of TG, TC, LDL-C and number of lipid droplets. Western blot results showed that the above beneficial effects could be achieved by regulating the expression of p-AMPK/ AMPK, SREBP1, FAS, ACC, SCD protein. Conclusion: This study confirmed the effect of STE on improving NAFLD and the potential action mechanism was involved in the regulation of the AMPK-SREBP1 pathway. |
| 源URL | [http://210.75.249.4/handle/363003/61976]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Ren, Lichengcheng,Wang, Ruinan,Wang, Yue,et al. Exploring the effect and mechanism of Hippophae rhamnoides L. triterpenoid acids on improving NAFLD based on network pharmacology and experimental validation in vivo and in vitro[J]. JOURNAL OF ETHNOPHARMACOLOGY,2024,335. |
| APA | Ren, Lichengcheng.,Wang, Ruinan.,Wang, Yue.,Tie, Fangfang.,Dong, Qi.,...&Hu, Na.(2024).Exploring the effect and mechanism of Hippophae rhamnoides L. triterpenoid acids on improving NAFLD based on network pharmacology and experimental validation in vivo and in vitro.JOURNAL OF ETHNOPHARMACOLOGY,335. |
| MLA | Ren, Lichengcheng,et al."Exploring the effect and mechanism of Hippophae rhamnoides L. triterpenoid acids on improving NAFLD based on network pharmacology and experimental validation in vivo and in vitro".JOURNAL OF ETHNOPHARMACOLOGY 335(2024). |
入库方式： OAI收割
来源：西北高原生物研究所
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