Creating a zero amylose barley with high soluble sugar content by genome editing
文献类型：期刊论文
| 作者 | Li, Yun; Jiang, Yanyan; Cao, Dong; Dang, Bin; Yang, Xijuan; Fan, Shiting; Shen, Yuhu ; Li, Genying; Liu, Baolong
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| 刊名 | PLANT MOLECULAR BIOLOGY
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| 出版日期 | 2024 |
| 卷号 | 114期号:3 |
| 英文摘要 | Amylose biosynthesis is strictly associated with granule-bound starch synthase I (GBSSI) encoded by the Waxy gene. Mutagenesis of single bases in the Waxy gene, which induced by CRISPR/Cas9 genome editing, caused absence of intact GBSSI protein in grain of the edited line. The amylose and amylopectin contents of waxy mutants were zero and 31.73%, while those in the wild type were 33.50% and 39.00%, respectively. The absence of GBSSI protein led to increase in soluble sugar content to 37.30% compared with only 10.0% in the wild type. Sucrose and beta-glucan, were 39.16% and 35.40% higher in waxy mutants than in the wild type, respectively. Transcriptome analysis identified differences between the wild type and waxy mutants that could partly explain the reduction in amylose and amylopectin contents and the increase in soluble sugar, sucrose and beta-glucan contents. This waxy flour, which showed lower final viscosity and setback, and higher breakdown, could provide more option for food processing.The new zero amylose barley was obtained via the CRISPR/Cas9 genome editing, showing higher soluble sugar content and lower final viscosity and setback. |
| 源URL | [http://210.75.249.4/handle/363003/62017]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Li, Yun,Jiang, Yanyan,Cao, Dong,et al. Creating a zero amylose barley with high soluble sugar content by genome editing[J]. PLANT MOLECULAR BIOLOGY,2024,114(3). |
| APA | Li, Yun.,Jiang, Yanyan.,Cao, Dong.,Dang, Bin.,Yang, Xijuan.,...&Liu, Baolong.(2024).Creating a zero amylose barley with high soluble sugar content by genome editing.PLANT MOLECULAR BIOLOGY,114(3). |
| MLA | Li, Yun,et al."Creating a zero amylose barley with high soluble sugar content by genome editing".PLANT MOLECULAR BIOLOGY 114.3(2024). |
入库方式： OAI收割
来源：西北高原生物研究所
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