Development and characterization of novel EST-SSR markers for Gentiana straminea Maxim., a traditional Tibetan herb in China and cross-amplification in related species
文献类型：期刊论文
| 作者 | Cheng, Tingfeng; Lin, Pengcheng; Zhou, Dangwei ; Wang, Huan; Shi, Shengbo; Shen, Jianwei; Meng, Jing; Ye, Xing; Zheng, Kun; Hu, Xingqiang
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| 刊名 | PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION
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| 出版日期 | 2024 |
| 英文摘要 | Gentiana straminea Maxim. (Gentianaceae) is an important traditional Tibetan herb that is mainly distributed on the Qinghai-Tibetan Plateau. Despite its agricultural and pharmacological importance, there remains a paucity of microsatellite markers, particularly expressed sequence tag-simple sequence repeat (EST-SSR) markers, available for this local endemic species. In this study, based on previous Illumina transcriptome data of G. straminea, a total of 96 EST-SSR markers were initially designed and tested. Thirty-two of 96 loci (33.33%) were successfully amplified and verified for validation. Among them, 10 were polymorphic and had clear bands. The polymorphism information content values were 0.09-0.799, the number of alleles per locus ranged from 3 to 14, and the levels of observed and expected heterozygosity were 0.078-0.722 and 0.238-0.884, respectively, which suggested a high level of information. Moreover, cross-amplification was successful for 10 loci in two other related species, Gentiana macrophylla Pallas and Gentiana dahurica Fischer. These EST-SSR markers provide a valuable tool for investigating the genetic diversity related to quantitative traits and population genetic studies on G. straminea and related species in sect. Cruciata Gaudin. |
| 源URL | [http://210.75.249.4/handle/363003/62036]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Cheng, Tingfeng,Lin, Pengcheng,Zhou, Dangwei,et al. Development and characterization of novel EST-SSR markers for Gentiana straminea Maxim., a traditional Tibetan herb in China and cross-amplification in related species[J]. PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION,2024. |
| APA | Cheng, Tingfeng.,Lin, Pengcheng.,Zhou, Dangwei.,Wang, Huan.,Shi, Shengbo.,...&Zhuang, Yuanwen.(2024).Development and characterization of novel EST-SSR markers for Gentiana straminea Maxim., a traditional Tibetan herb in China and cross-amplification in related species.PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION. |
| MLA | Cheng, Tingfeng,et al."Development and characterization of novel EST-SSR markers for Gentiana straminea Maxim., a traditional Tibetan herb in China and cross-amplification in related species".PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION (2024). |
入库方式： OAI收割
来源：西北高原生物研究所
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