Innovative orthogonal two-dimensional reversed-phase liquid chromatography x supercritical fluid chromatography with a phenyl/ tetrazole stationary phase for the preparative isolation of diarylheptanoids
文献类型：期刊论文
| 作者 | Dang, Jun; Tong, Yingying; Wang, Qilan ; Li, Gang; Abd El-Aty, A. M.
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| 刊名 | JOURNAL OF CHROMATOGRAPHY A
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| 出版日期 | 2024 |
| 卷号 | 1726 |
| 英文摘要 | In this investigation, we successfully isolated and purified natural diarylheptanoids using an orthogonal offline two-dimensional RPLC x SFC approach, employing only the phenyl/tetrazole stationary phase. First, a styrenedivinylbenzene matrix medium pretreatment liquid chromatography system effectively processed chlorophyllcontaining plant extract solution with a recovery rate of 33.8 %, obviating the need for concentration steps. Subsequently, an offline two-dimensional RPLC x SFC employing only the phenyl/tetrazole stationary phase achieved a remarkable 96.38 % orthogonality and was established and utilized in the preparative separation and purification of natural products. Finally, the constructed single stationary phase highly orthogonal RPLC x SFC system was successfully applied in the preparative separation and purification of natural diarylheptanoids from the Saxifraga tangutica target fraction and yielded four diarylheptanoids with purities exceeding 95 %. |
| 源URL | [http://210.75.249.4/handle/363003/62039]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Dang, Jun,Tong, Yingying,Wang, Qilan,et al. Innovative orthogonal two-dimensional reversed-phase liquid chromatography x supercritical fluid chromatography with a phenyl/ tetrazole stationary phase for the preparative isolation of diarylheptanoids[J]. JOURNAL OF CHROMATOGRAPHY A,2024,1726. |
| APA | Dang, Jun,Tong, Yingying,Wang, Qilan,Li, Gang,&Abd El-Aty, A. M..(2024).Innovative orthogonal two-dimensional reversed-phase liquid chromatography x supercritical fluid chromatography with a phenyl/ tetrazole stationary phase for the preparative isolation of diarylheptanoids.JOURNAL OF CHROMATOGRAPHY A,1726. |
| MLA | Dang, Jun,et al."Innovative orthogonal two-dimensional reversed-phase liquid chromatography x supercritical fluid chromatography with a phenyl/ tetrazole stationary phase for the preparative isolation of diarylheptanoids".JOURNAL OF CHROMATOGRAPHY A 1726(2024). |
入库方式： OAI收割
来源：西北高原生物研究所
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