Fortified Dual-Spectral Overlap with Enhanced Colorimetric/Fluorescence Dual-Response Immunochromatography for On-Site Bimodal-Type Gentamicin Monitoring
文献类型：期刊论文
| 作者 | Liu, Sijie; Zhao, Cong; Shu, Rui; Dou, Leina; Luo, Xing; Luo, Linpin; Sun, Jing; Wang, Yanru; Ji, Yanwei; Wang, Jianlong |
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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| 出版日期 | 2024 |
| 卷号 | 72期号:16 |
| 英文摘要 | Immunochromatography (ICA) remains untapped toward enhanced sensitivity and applicability for fulfilling the nuts and bolts of on-site food safety surveillance. Herein, we report a fortified dual-spectral overlap with enhanced colorimetric/fluorescence dual-response ICA for on-site bimodal-type gentamicin (Gen) monitoring by employing polydopamine (PDA)-coated AuNPs (APDA) simultaneously serving as a colorimetric reporter and a fluorescence quencher. Availing of the enhanced colorimetric response that originated from the PDA layer, the resultant APDA exhibits less required antibody and immunoprobes in a single immunoassay, which facilitates improved antibody utilization efficiency and immuno-recognition in APDA-ICA. Further integrated with the advantageous features of fortified excitation and emission dual-spectral overlap for the Arg/ATT-AuNCs, this APDA-ICA with a turn on/off pattern achieves the visual limits of detection of 1.0 and 0.5 ng mL(-1) for colorimetric and fluorescence patterns (25- and 50-fold lower than standard AuNPs-ICA). Moreover, the excellent self-calibration and satisfactory recovery of 79.03-118.04% were shown in the on-site visual colorimetric-fluorescence analysis for Gen in real environmental media (including real river water, an urban aquaculture water body, an aquatic product, and an animal byproduct). This work provides the feasibility of exploiting fortified dual-spectral overlap with an enhanced colorimetric/fluorescence dual response for safeguarding food safety and public health. |
| 源URL | [http://210.75.249.4/handle/363003/62048]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Liu, Sijie,Zhao, Cong,Shu, Rui,et al. Fortified Dual-Spectral Overlap with Enhanced Colorimetric/Fluorescence Dual-Response Immunochromatography for On-Site Bimodal-Type Gentamicin Monitoring[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2024,72(16). |
| APA | Liu, Sijie.,Zhao, Cong.,Shu, Rui.,Dou, Leina.,Luo, Xing.,...&Wang, Jianlong.(2024).Fortified Dual-Spectral Overlap with Enhanced Colorimetric/Fluorescence Dual-Response Immunochromatography for On-Site Bimodal-Type Gentamicin Monitoring.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,72(16). |
| MLA | Liu, Sijie,et al."Fortified Dual-Spectral Overlap with Enhanced Colorimetric/Fluorescence Dual-Response Immunochromatography for On-Site Bimodal-Type Gentamicin Monitoring".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72.16(2024). |
入库方式： OAI收割
来源：西北高原生物研究所
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