Lichen pectin-containing polysaccharide from Xanthoria elegans and its ability to effectively protect LX-2 cells from H 2 O 2-induced oxidative damage
文献类型：期刊论文
| 作者 | Zhou, Zheng; Li, Guoqiang; Gao, Liang; Zhou, Yubi; Xiao, Yuancan; Bi, Hongtao; Yang, Hongxia |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2024 |
| 卷号 | 265 |
| 英文摘要 | Xanthoria elegans , a drought -tolerant lichen, is the original plant of the traditional Chinese medicine Shihua and effectively treats a variety of liver diseases. However, thus far, the hepatoprotective effects of polysaccharides, the most important chemical constituents of X. elegans , have not been determined. The aim of this study was to screen the polysaccharide fraction for hepatoprotective activity by using free radical scavenging assays and a H 2 O 2 -induced Lieming Xu-2 cell (LX -2) oxidative damage model and to elucidate the chemical composition of the bioactive polysaccharide fraction. In the present study, three polysaccharide fractions (XEP-50, XEP-70 and XEP90) were obtained from X. elegans by hot-water extraction, DEAE-cellulose anion exchange chromatography separation and ethanol gradient precipitation. Among the three polysaccharide fractions, XEP-70 exhibited the best antioxidant activity in free radical scavenging capacity and reducing power assays. Structural studies showed that XEP-70 was a pectin -containing heteropolysaccharide fraction that was composed mainly of (1 -> 4) -linked and (1 -> 4,6) -linked alpha-D-Glc p , (1 -> 4) -linked alpha-D-Gal p A, (1 -> 2) -linked, (1 -> 6) -linked and (1 -> 2,6)linked alpha-D-Man p , and (1 -> 6) -linked and (1 -> 2,6) -linked beta- D-Gal f . Furthermore, XEP-70 exhibited effectively protect LX -2 cells against H 2 O 2 -induced oxidative damage by enhancing cellular antioxidant capacity by activating the Nrf2/Keap1/ARE signaling pathway. Thus, XEP-70 has good potential to protect hepatic stellate cells against oxidative damage. |
| 源URL | [http://210.75.249.4/handle/363003/62066]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Zhou, Zheng,Li, Guoqiang,Gao, Liang,et al. Lichen pectin-containing polysaccharide from Xanthoria elegans and its ability to effectively protect LX-2 cells from H 2 O 2-induced oxidative damage[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,265. |
| APA | Zhou, Zheng.,Li, Guoqiang.,Gao, Liang.,Zhou, Yubi.,Xiao, Yuancan.,...&Yang, Hongxia.(2024).Lichen pectin-containing polysaccharide from Xanthoria elegans and its ability to effectively protect LX-2 cells from H 2 O 2-induced oxidative damage.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,265. |
| MLA | Zhou, Zheng,et al."Lichen pectin-containing polysaccharide from Xanthoria elegans and its ability to effectively protect LX-2 cells from H 2 O 2-induced oxidative damage".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 265(2024). |
入库方式： OAI收割
来源：西北高原生物研究所
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