Target-guided isolation and purification of cyclooxygenase-2 inhibitors from Meconopsis integrifolia (Maxim.) Franch. by high-speed counter-current chromatography combined with ultrafiltration liquid chromatography
文献类型：期刊论文
| 作者 | Li, Aijing; La, Mencuo; Wang, Yao; Chen, Tao; Mian, Ruisha; He, Fangfang; Li, Yulin ; Zou, Denglang
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| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2024 |
| 卷号 | 47期号:1 |
| 英文摘要 | Meconopsis integrifolia (Maxim.) Franch. is used extensively in traditional Tibetan medicine for its potent anti-inflammatory properties. In this study, six cyclooxygenase-2 (COX-2) inhibitors were purified from M. integrifolia using high-speed counter-current chromatography guided by ultrafiltration liquid chromatography (ultrafiltration-LC). First, ultrafiltration-LC was performed to profile the COX-2 inhibitors in M. integrifolia. The reflux extraction conditions were further optimized using response surface methodology, and the results showed that the targeted COX-2 inhibitors could be well enriched under the optimized extraction conditions. Then the six target COX-2 inhibitors were separated by high-speed countercurrent chromatography with a solvent system composed of ethyl acetate/n-butanol/water (4:1:4, v/v/v. Finally, the six COX-2 inhibitors, including 21.2 mg of 8-hydroxyluteolin 7-sophoroside, 29.6 mg of 8-hydroxyluteolin 7-[6 '''-acetylallosyl-(1 -> 2)-glucoside], 42.5 mg of Sinocrassoside D3, 54.1 mg of Hypolaetin 7-[6 '''-acetylallosyll-(l -> 2)-3 ''-acetylglucoside, 30.6 mg of Hypolaetin 7-[6 '''-acetylallosyll-(l -> 2)-6 ''-acetylglucoside and 17.8 mg of Hypolaetin were obtained from 500 mg of sample. Their structures were elucidated by H-1-NMR spectroscopy. This study reveals that ultrafiltration-LC combined with high-speed counter-current chromatography is a robust and efficient strategy for target-guided isolation and purification of bioactive molecules. It also enhances the scientific understanding of the anti-inflammatory properties of M. integrifolia but also paves the way for its further medicinal applications. |
| 源URL | [http://210.75.249.4/handle/363003/62118]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Li, Aijing,La, Mencuo,Wang, Yao,et al. Target-guided isolation and purification of cyclooxygenase-2 inhibitors from Meconopsis integrifolia (Maxim.) Franch. by high-speed counter-current chromatography combined with ultrafiltration liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2024,47(1). |
| APA | Li, Aijing.,La, Mencuo.,Wang, Yao.,Chen, Tao.,Mian, Ruisha.,...&Zou, Denglang.(2024).Target-guided isolation and purification of cyclooxygenase-2 inhibitors from Meconopsis integrifolia (Maxim.) Franch. by high-speed counter-current chromatography combined with ultrafiltration liquid chromatography.JOURNAL OF SEPARATION SCIENCE,47(1). |
| MLA | Li, Aijing,et al."Target-guided isolation and purification of cyclooxygenase-2 inhibitors from Meconopsis integrifolia (Maxim.) Franch. by high-speed counter-current chromatography combined with ultrafiltration liquid chromatography".JOURNAL OF SEPARATION SCIENCE 47.1(2024). |
入库方式： OAI收割
来源：西北高原生物研究所
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