Goat anti-mouse immunoglobulin as crosslinker assisted signal tracer assemble with intensive antibody utilization efficiency for sensitive paper-based strip nanobiosensors
文献类型：期刊论文
| 作者 | Liu, Sijie; Shu, Rui; Zhang, Mingrui; Zhao, Cong; Wang, Kexin; Zhang, Jiayi; Sun, Jing; Dou, Leina; Zhang, Daohong; Wang, Jianlong |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2024 |
| 卷号 | 258 |
| 英文摘要 | Engineered collaborative biochemical techniques and regulated nanomaterials (NMs) offer extraordinary opportunities for improving the analysis performance of lateral flow immunoassay (LFIA). Herein, inspired by the ability of macromolecules (e.g., proteins) to assemble into new functional units and the remarkable optical performance of engineered regulated NMs, goat anti-mouse immunoglobulin (GAMI) serves as the crosslinker integrate with gold-manganese oxide (Au-MnOx) to assemble the signal tracers (STs)-crosslinker-antibody (mAb) for elevating the mAb utilization efficiency. Notably, the STs-crosslinker-mAb assembly shows -13.33folds mAb utilization efficiency enhance, which perfectly response the challenge between limited sensitivity and sufficient signal intensity in competitive-type LFIA. The black color and rough structure of Au-MnOx offer higher colorimetric brightness (-2-folds than AuNPs) and enhanced mAb coupling efficiency (up to 92.47%), which further improves sensitivity under the premise of functional assembly to intensify the competitive immunoreaction. Additionally, the convenient synthesis conditions (-13 min at room temperature) even comparable to direct purchase commercial products indicate that using Au-MnOx undoubtedly increases the cost-effectiveness. Encouragingly, the Au-MnOx-GAMI-mAb based LFIA exhibited high sensitivity (LOD: 0.063 ng mL-1 for clenbuterol (CLE) monitoring) by elevating mAb utilization efficiency with the attendant enhancing immune competition response in a cost-effective manner, which provides an invigorating reference pathway in point-ofcare immunoassay. |
| 源URL | [http://210.75.249.4/handle/363003/62123]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Liu, Sijie,Shu, Rui,Zhang, Mingrui,et al. Goat anti-mouse immunoglobulin as crosslinker assisted signal tracer assemble with intensive antibody utilization efficiency for sensitive paper-based strip nanobiosensors[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,258. |
| APA | Liu, Sijie.,Shu, Rui.,Zhang, Mingrui.,Zhao, Cong.,Wang, Kexin.,...&Wang, Jianlong.(2024).Goat anti-mouse immunoglobulin as crosslinker assisted signal tracer assemble with intensive antibody utilization efficiency for sensitive paper-based strip nanobiosensors.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,258. |
| MLA | Liu, Sijie,et al."Goat anti-mouse immunoglobulin as crosslinker assisted signal tracer assemble with intensive antibody utilization efficiency for sensitive paper-based strip nanobiosensors".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 258(2024). |
入库方式： OAI收割
来源：西北高原生物研究所
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