The transporter PHO84/NtPT1 is a target of aluminum to affect phosphorus absorption in Saccharomyces cerevisiae and Nicotiana tabacum L.
文献类型：期刊论文
| 作者 | Huang, Zhiwei; Zhang, Shixuan; Chen, Ranran; Zhu, Qian; Shi, Ping; Shen, Yuhu
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| 刊名 | METALLOMICS
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| 出版日期 | 2023 |
| 卷号 | 15期号:12 |
| 英文摘要 | The molecular mechanism of aluminum toxicity in biological systems is not completely understood. Saccharomyces cerevisiae is one of the most used model organisms in the study of environmental metal toxicity. Using an unbiased metallomic approach in yeast, we found that aluminum treatment caused phosphorus deprivation, and the lack of phosphorus increased as the pH of the environment decreased compared to the control strain. By screening the phosphate signaling and response pathway (PHO pathway) in yeast with the synthetic lethality of a new phosphorus-restricted aluminum-sensitive gene, we observed that pho84 Delta mutation conferred severe growth defect to aluminum under low-phosphorus conditions, and the addition of phosphate alleviated this sensitivity. Subsequently, the data showed that PHO84 determined the intracellular aluminum-induced phosphorus deficiency, and the expression of PHO84 was positively correlated with aluminum stress, which was mediated by phosphorus through the coordinated regulation of PHO4/PHO2. Moreover, aluminum reduced phosphorus absorption and inhibited tobacco plant growth in acidic media. In addition, the high-affinity phosphate transporter NtPT1 in tobacco exhibited similar effects to PHO84, and overexpression of NtPT1 conferred aluminum resistance in yeast cells. Taken together, positive feedback regulation of the PHO pathway centered on the high-affinity phosphate transporters is a highly conservative mechanism in response to aluminum toxicity. The results may provide a basis for aluminum-resistant microorganisms or plant engineering and acidic soil treatment.Graphical Abstract PHO84 is a target of aluminum stress. |
| 源URL | [http://210.75.249.4/handle/363003/62129]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Huang, Zhiwei,Zhang, Shixuan,Chen, Ranran,et al. The transporter PHO84/NtPT1 is a target of aluminum to affect phosphorus absorption in Saccharomyces cerevisiae and Nicotiana tabacum L.[J]. METALLOMICS,2023,15(12). |
| APA | Huang, Zhiwei,Zhang, Shixuan,Chen, Ranran,Zhu, Qian,Shi, Ping,&Shen, Yuhu.(2023).The transporter PHO84/NtPT1 is a target of aluminum to affect phosphorus absorption in Saccharomyces cerevisiae and Nicotiana tabacum L..METALLOMICS,15(12). |
| MLA | Huang, Zhiwei,et al."The transporter PHO84/NtPT1 is a target of aluminum to affect phosphorus absorption in Saccharomyces cerevisiae and Nicotiana tabacum L.".METALLOMICS 15.12(2023). |
入库方式： OAI收割
来源：西北高原生物研究所
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