Hirsutella sinensis mycelium polysaccharides attenuate the TGF-β1-induced epithelial-mesenchymal transition in human intrahepatic bile duct epithelial cells
文献类型：期刊论文
| 作者 | Yang, Mengmeng; Rong, Lin; Zhang, Xingfang; Li, Guoqiang; Wang, Qiannan; Li, Cen; Xiao, Yuancan; Wei, Lixin ; Bi, Hongtao
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| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| 出版日期 | 2024 |
| 卷号 | 254 |
| 英文摘要 | Hirsutella sinensis is the anamorph of Ophiocordyceps sinensis, and its mycelia has been used to effectively treat a variety of hepatobiliary diseases in clinical practice. In the present study, we performed a systematic study on the composition and structure of its polysaccharides, and then employed a TGF-beta 1-induced human intrahepatic bile duct epithelial cell-epithelial-mesenchymal transition (HIBEC-EMT) model to investigate their effects on treating primary biliary cholangitis (PBC) based on hepatic bile duct fibrosis. Four polysaccharide fractions were obtained from H. sinensis mycelia by hot-water extraction, DEAE-cellulose column and gradient ethanol precipitation separation. HSWP-1a was an alpha-(1,4)-D-glucan; HSWP-1b and HSWP-1d mainly consisted of mannoglucans with a backbone composed of 1,4-linked alpha-D-Glcp and 1,4,6-linked alpha-D-Manp residues branched at O-6 of the 1,4-linked alpha-D-Glcp with a 1-linked alpha-D-Glcp as a side chain; and HSWP-1c mainly contained galactomannoglucans. These polysaccharide fractions protected HIBECs from a TGF-beta 1-induced EMT, according to HIBEC morphological changes, cell viability, decreased E-cadherin and ZO-1 expression, and increased vimentin and collagen I expression. Furthermore, the effects of the polysaccharides might be mediated by inhibiting the activation of the TGF-beta/Smad signaling pathway, which attenuated hepatic bile duct fibrosis and potential PBC effects. |
| 源URL | [http://210.75.249.4/handle/363003/62162]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Yang, Mengmeng,Rong, Lin,Zhang, Xingfang,et al. Hirsutella sinensis mycelium polysaccharides attenuate the TGF-β1-induced epithelial-mesenchymal transition in human intrahepatic bile duct epithelial cells[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,254. |
| APA | Yang, Mengmeng.,Rong, Lin.,Zhang, Xingfang.,Li, Guoqiang.,Wang, Qiannan.,...&Bi, Hongtao.(2024).Hirsutella sinensis mycelium polysaccharides attenuate the TGF-β1-induced epithelial-mesenchymal transition in human intrahepatic bile duct epithelial cells.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,254. |
| MLA | Yang, Mengmeng,et al."Hirsutella sinensis mycelium polysaccharides attenuate the TGF-β1-induced epithelial-mesenchymal transition in human intrahepatic bile duct epithelial cells".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 254(2024). |
入库方式： OAI收割
来源：西北高原生物研究所
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