Anti-Idiotypic Nanobody Alkaline Phosphatase Fusion Protein-Triggered On-Off-On Fluorescence Immunosensor for Aflatoxin in Cereals
文献类型：期刊论文
| 作者 | Yu, Gege; Wang, Jiamin; Zhang, Yao; Wu, Haofen; Wang, Yueqi; Cui, Yan; Yang, Yuefan; Tang, Xiaoqian; Zhang, Qi; Wang, Jianlong |
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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| 出版日期 | 2023 |
| 卷号 | 71期号:45 |
| 英文摘要 | Nanobodies (Nbs) are widely used in immunoassays with the advantages of small size and high stability. Here, the nanobody employed as the surrogate of aflatoxin antigen and the recognition mechanism of antiaflatoxin mAb with nanobody was studied by molecular modeling, which verified the feasibility of Nbs as antigen substitutes. On this basis, a nanobody-alkaline phosphatase fusion protein (Nb-AP) was constructed, and a highly sensitive on-off-on fluorescent immunosensor (OFO-FL immunosensor) based on the calcein/Ce3+ system was developed for aflatoxin quantification. Briefly, calcein serves as a signal transducer, and its fluorescence can be quenched after it is bound with Ce3+. In the presence of Nb-AP, AP catalyzed p-nitrophenyl phosphate to generate orthophosphate, which competes in binding with Ce3+, leading to fluorescence recovery. The method has a linearity range of 0.005-100 ng/mL, and the IC50 of the OFO-FL immunosensor was 0.063 ng/mL, which was 18-fold lower than that of conventional enzyme-linked immunosorbent assay. The assay was successfully applied in food samples with a recovery of 88-121%. |
| 源URL | [http://210.75.249.4/handle/363003/62166]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Yu, Gege,Wang, Jiamin,Zhang, Yao,et al. Anti-Idiotypic Nanobody Alkaline Phosphatase Fusion Protein-Triggered On-Off-On Fluorescence Immunosensor for Aflatoxin in Cereals[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2023,71(45). |
| APA | Yu, Gege.,Wang, Jiamin.,Zhang, Yao.,Wu, Haofen.,Wang, Yueqi.,...&Li, Peiwu.(2023).Anti-Idiotypic Nanobody Alkaline Phosphatase Fusion Protein-Triggered On-Off-On Fluorescence Immunosensor for Aflatoxin in Cereals.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,71(45). |
| MLA | Yu, Gege,et al."Anti-Idiotypic Nanobody Alkaline Phosphatase Fusion Protein-Triggered On-Off-On Fluorescence Immunosensor for Aflatoxin in Cereals".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 71.45(2023). |
入库方式： OAI收割
来源：西北高原生物研究所
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