Acetyl coenzyme A carboxylase modulates lipogenesis and sugar homeostasis in Blattella germanica
文献类型：期刊论文
| 作者 | Pei, Xiaojin; Bai, Tiantian; Luo, Yuan; Zhang, Zhanfeng; Li, Sheng; Fan, Yongliang; Liu, Tong-Xian |
| 刊名 | INSECT SCIENCE
 |
| 出版日期 | 2024 |
| 卷号 | 31期号:2 |
| 英文摘要 | Lipid and sugar homeostasis is critical for insect development and survival. In this study, we characterized an acetyl coenzyme A carboxylase gene in Blattella germanica (BgACC) that is involved in both lipogenesis and sugar homeostasis. We found that BgACC was dominantly expressed in the fat body and integument, and was significantly upregulated after molting. Knockdown of BgACC in 5th-instar nymphs did not affect their normal molting to the next nymphal stage, but it caused a lethal phenotype during adult emergence. BgACC-RNA interference (RNAi) significantly downregulated total free fatty acid (FFA) and triacylglycerol (TAG) levels, and also caused a significant decrease of cuticular hydrocarbons (CHCs). Repression of BgACC in adult females affected the development of oocytes and resulted in sterile females, but BgACC-RNAi did not affect the reproductive ability of males. Interestingly, knockdown of BgACC also changed the expression of insulin-like peptide genes (BgILPs), which mimicked a physiological state of high sugar uptake. In addition, BgACC was upregulated when B. germanica were fed on a high sucrose diet, and repression of BgACC upregulated the expression of the glycogen synthase gene (BgGlyS). Moreover, BgACC-RNAi increased the circulating sugar levels and glycogen storage, and a longevity assay suggested that BgACC was important for the survival of B. germanica under conditions of high sucrose uptake. Our results confirm that BgACC is involved in multiple lipid biogenesis and sugar homeostasis processes, which further modulates insect reproduction and sugar tolerance. This study benefits our understanding of the crosstalk between lipid and sugar metabolism. |
| 源URL | [http://210.75.249.4/handle/363003/62175]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Pei, Xiaojin,Bai, Tiantian,Luo, Yuan,et al. Acetyl coenzyme A carboxylase modulates lipogenesis and sugar homeostasis in Blattella germanica[J]. INSECT SCIENCE,2024,31(2). |
| APA | Pei, Xiaojin.,Bai, Tiantian.,Luo, Yuan.,Zhang, Zhanfeng.,Li, Sheng.,...&Liu, Tong-Xian.(2024).Acetyl coenzyme A carboxylase modulates lipogenesis and sugar homeostasis in Blattella germanica.INSECT SCIENCE,31(2). |
| MLA | Pei, Xiaojin,et al."Acetyl coenzyme A carboxylase modulates lipogenesis and sugar homeostasis in Blattella germanica".INSECT SCIENCE 31.2(2024). |
入库方式： OAI收割
来源：西北高原生物研究所
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