High-Level Production of Recombinant Lipase by Fed-Batch Fermentation in Escherichia coli and Its Application in Biodiesel Synthesis from Waste Cooking Oils
文献类型：期刊论文
| 作者 | Zhang, Jun2,3,4; Luo, Wen3 ; Wang, Zhiyuan3; Chen, Yiaoyan3; Fu, Junying3; Xu, Jingliang1; Lv, Pengmei3
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| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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| 出版日期 | 2022-09-10 |
| 页码 | 19 |
| 关键词 | Lipase Escherichia coli Fermentation Biodiesel Waste cooking oils |
| ISSN号 | 0273-2289 |
| DOI | 10.1007/s12010-022-04146-6 |
| 通讯作者 | Wang, Zhiyuan(wangzy@ms.giec.ac.cn) ; Lv, Pengmei(lvpm@ms.giec.ac.cn) |
| 英文摘要 | The enzymatic production of biodiesel from waste cooking oils (WCOs) offers a green and sustainable solution for the liquid fuel manufacture as well as waste resource recovery. In present study, liquid lipase was used to simplify the catalysis process, thereby reducing biodiesel production costs. An engineered Escherichia coli expressing Geobacillus thermocatenulatus lipase 2 (GTL2) was screened at an enzyme activity of 6.96 U/mg, after evaluating the propagating stability of the recombinant plasmids exceeding 86.11%. Through the beneficial feeding strategy and effective pH control, high-level production of GTL2 by fed-batch fermentation was achieved with an enzyme activity of 434.32 U/mg, which was almost 62 times that of shake flask fermentation. In addition, liquid GTL2 was used to prepare fatty acid methyl esters (FAMEs) using WCOs. The effects of the reaction time, catalyst loading, temperature, and methanol-to-oil molar ratio on FAMEs production using WCOs were explored, and a maximum FAMEs yield of 96.62% was achieved under optimized conditions. These results indicate that liquid GTL2 is a promising biocatalyst for efficient utilization of WCOs in the synthesis of biodiesel and provide a novel enzymatic process for biodiesel reducing the cost of production. |
| WOS关键词 | PLASMID STABILITY ; PALM OIL ; TRANSESTERIFICATION ; CONVERSION ; EXPRESSION |
| 资助项目 | Natural Science Foundation of Guangdong Province of China[2020A1515010513] ; Program for Scientific Research Start-up Funds of Guangdong Ocean University[060302042110] ; National Natural Science Foundation of China[51903236] ; National Natural Science Foundation of China[51606201] ; Projects of International Cooperation and Exchanges NSFC[51861145103] ; Program of CAS Key Laboratory of Renewable Energy[E029010801] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000852357400002 |
| 出版者 | SPRINGER |
| 资助机构 | Natural Science Foundation of Guangdong Province of China ; Program for Scientific Research Start-up Funds of Guangdong Ocean University ; National Natural Science Foundation of China ; Projects of International Cooperation and Exchanges NSFC ; Program of CAS Key Laboratory of Renewable Energy |
| 源URL | [http://ir.giec.ac.cn/handle/344007/37251]  |
| 专题 | 中国科学院广州能源研究所 |
| 通讯作者 | Wang, Zhiyuan; Lv, Pengmei |
| 作者单位 | 1.Zhengzhou Univ, Sch Chem Engn, Zhengzhou 450001, Peoples R China 2.Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China 3.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China 4.Guangdong Ocean Univ, Coll Food Sci & Technol,Guangdong Prov Engn Lab M, Guangdong Prov Engn Technol Res Ctr Seafood, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Zhanjiang 524088, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jun,Luo, Wen,Wang, Zhiyuan,et al. High-Level Production of Recombinant Lipase by Fed-Batch Fermentation in Escherichia coli and Its Application in Biodiesel Synthesis from Waste Cooking Oils[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2022:19. |
| APA | Zhang, Jun.,Luo, Wen.,Wang, Zhiyuan.,Chen, Yiaoyan.,Fu, Junying.,...&Lv, Pengmei.(2022).High-Level Production of Recombinant Lipase by Fed-Batch Fermentation in Escherichia coli and Its Application in Biodiesel Synthesis from Waste Cooking Oils.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,19. |
| MLA | Zhang, Jun,et al."High-Level Production of Recombinant Lipase by Fed-Batch Fermentation in Escherichia coli and Its Application in Biodiesel Synthesis from Waste Cooking Oils".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2022):19. |
入库方式： OAI收割
来源：广州能源研究所
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