G-Quadruplex Dimer/Exonuclease I Assisted Signal Amplification Strategy for Rapid Determination of Aflatoxin B1 Using a Paper Chip
文献类型：期刊论文
| 作者 | He, Xuan1; Qi, Ji4; Yu, Zi-Hui2; Chen, Yan3; Fu, Xiu-Li1 |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2024-08-01 |
| 卷号 | 52期号:8页码:1094-1102 |
| 关键词 | Rotational paper-based analytical device Exonuclease I G-quadruplex dimer Tetrahedral DNA nanostructure Aflatoxin B1 |
| ISSN号 | 0253-3820 |
| DOI | 10.19756/j.issn.0253-3820.231149 |
| 通讯作者 | Fu, Xiu-Li() |
| 英文摘要 | In this work, a tetrahedral DNA nanostructure (TDN) functionalized rotational paper-based analytical device (RPAD) was constructed for rapid and highly sensitive detection of aflatoxin B1 (AFB1) using exonuclease I (Exo I ) and G-quadruplex (G4) dimer. Herein, a single-stranded DNA, containing both of the G4 dimer sequence and AFB1 recognition sequence, was used as the recognition probe (G4 dimer probe). TDN was used to precisely regulate the orientation and distribution density of G4 dimer probe to improve the recognition efficiency of the system. Exo I as a single stranded DNA specific nuclease was introduced for effective amplification of the detection signal. G4 dimer was employed to enhance the fluorescence signal of thioflavin T (ThT). In the absence of AFB1, the G4 dimer structure of G4 dimer probe could specifically bind with ThT to generate dramatic fluorescence enhancement. However, in the presence of AFB1, AFB1 could specifically bind with G4 dimer probe, resulting in the dissociation of G4 dimer probe from TDN and further be digested by Exo I . At the same time, the released AFB1 could bind to G4 dimer probe on the TDN again by this way to generate signal amplification. After this cycle, the amount of aptamer on the TDN was decreased, accompanied by the reduction of G4 dimer on TDN. In this case, the fluorescence intensity of the system was reduced. The designed RPAD showed a good linear response in AFB1 concentration range of 0.0001-500 ng/mL and the limit of detection was 0.1 pg/mL. Moreover, the proposed strategy was successfully applied to detection of AFB1 in peanut and wine. The developed TDN/G4 dimer/Exo I strategy improved the specificity and sensitivity of the system significantly. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; 16 KINDS ; MYCOTOXINS ; APTASENSOR ; B-1 |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001337991900001 |
| 资助机构 | Qingchuang Science and Technology Program of Shandong Province, China ; National Natural Science Foundation of China |
| 源URL | [http://ir.yic.ac.cn/handle/133337/37749]  |
| 专题 | 中国科学院烟台海岸带研究所 |
| 通讯作者 | Fu, Xiu-Li |
| 作者单位 | 1.Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China 2.Yantai Univ, Sch Phys & Elect Informat, Yantai 264005, Peoples R China 3.Shandong Agr & Engn Univ, Sch Resources & Environm Engn, Jinan 250100, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Xuan,Qi, Ji,Yu, Zi-Hui,et al. G-Quadruplex Dimer/Exonuclease I Assisted Signal Amplification Strategy for Rapid Determination of Aflatoxin B1 Using a Paper Chip[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2024,52(8):1094-1102. |
| APA | He, Xuan,Qi, Ji,Yu, Zi-Hui,Chen, Yan,&Fu, Xiu-Li.(2024).G-Quadruplex Dimer/Exonuclease I Assisted Signal Amplification Strategy for Rapid Determination of Aflatoxin B1 Using a Paper Chip.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,52(8),1094-1102. |
| MLA | He, Xuan,et al."G-Quadruplex Dimer/Exonuclease I Assisted Signal Amplification Strategy for Rapid Determination of Aflatoxin B1 Using a Paper Chip".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 52.8(2024):1094-1102. |
入库方式： OAI收割
来源：烟台海岸带研究所
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