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Oral administration of anti-lipopolysaccharide factor isoform 3 via the bait microalga for controlling bacterial infection of Atlantic Bay scallop ( Argopecten irradians)

文献类型：期刊论文

作者Cui, Yulin1,2,5; Liu, Ping4; Shao, Yaping1; Sun, Fengjie3; Wang, Qiaolei1; Zhang, Hao1; Cheng, Yan1; Gao, Zhengquan1
刊名AQUACULTURE REPORTS
出版日期2024-12-01
卷号39页码:10
关键词Anti-lipopolysaccharide factor isoform 3 Fusion expression Argopecten irradians Pathogenic bacteria
ISSN号2352-5134
DOI10.1016/j.aqrep.2024.102430
通讯作者Cui, Yulin(yulincui23@bzmc.edu.cn) ; Gao, Zhengquan(gaozhengquan@bzmc.edu.cn)
英文摘要In order to reduce antibiotic application in aquaculture, an antimicrobial peptide anti-lipopolysaccharide factor isoform 3 (ALFPm3) with highly effective inhibitory activity against pathogens was introduced to bay scallop (Argopecten irradians) using bait microalga Tetraselmis subcordiformis as a delivery system. To improve its expression and stability, a fusion of two alfpm3 genes (alfpm3-2) was inserted into an expression vector and transformed into T. subcordiformis chloroplast, followed by in vitro and in vivo functional evaluations of the antimicrobial activities of ALFPm3-2 by minimum inhibitory concentration method and bay scallop breeding experiment, respectively. The results showed that the transformants of T. subcordiformis and the purified ALFPm3-2 had significant inhibitory effects on Vibrio parahaemolyticus and Staphylococcus aureus in vitro, while feeding the transformants reduced the colonization of the two pathogens in bay scallops, which further reduced bay scallops' mortality by 28.67 % as revealed in infection test. Furthermore, daily oral administration of ALFPm3-2 could improve the gut microbial community and reduce the inflammatory response. Combined with changes in immune factors and endotoxin, it indicated that exogenous ALFPm3-2 played antimicrobial and antiinflammatory functions in the guts of bay scallops. Overall, this study provided strong evidence to support the oral administration of antimicrobial peptides via microalgae in aquaculture.
WOS关键词ANTI-LPS FACTOR ; PENAEUS-MONODON ; HORSESHOE-CRAB ; EXPRESSION ; PROTEIN ; GENE ; RESISTANCE ; ALFPM3
WOS研究方向Fisheries
语种英语
WOS记录号WOS:001339443000001
资助机构National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Binzhou Medical University
源URL[http://ir.yic.ac.cn/handle/133337/37792]  
专题海岸带生物学与生物资源利用重点实验室
烟台海岸带研究所_中科院烟台海岸带研究所知识产出
通讯作者Cui, Yulin; Gao, Zhengquan
作者单位1.Binzhou Med Univ, Sch Pharm, Yantai 264003, Shandong, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Shandong, Peoples R China
3.Georgia Gwinnett Coll, Sch Sci & Technol, Dept Biol Sci, Lawrenceville, GA 30043 USA
4.Yantai Univ, Coll Life Sci, Yantai 264003, Shandong, Peoples R China
5.Shandong Univ Tradit Chinese Med, Qingdao Acad Chinese Med Sci, Qingdao 266112, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Cui, Yulin,Liu, Ping,Shao, Yaping,et al. Oral administration of anti-lipopolysaccharide factor isoform 3 via the bait microalga for controlling bacterial infection of Atlantic Bay scallop ( Argopecten irradians)[J]. AQUACULTURE REPORTS,2024,39:10.
APA Cui, Yulin.,Liu, Ping.,Shao, Yaping.,Sun, Fengjie.,Wang, Qiaolei.,...&Gao, Zhengquan.(2024).Oral administration of anti-lipopolysaccharide factor isoform 3 via the bait microalga for controlling bacterial infection of Atlantic Bay scallop ( Argopecten irradians).AQUACULTURE REPORTS,39,10.
MLA Cui, Yulin,et al."Oral administration of anti-lipopolysaccharide factor isoform 3 via the bait microalga for controlling bacterial infection of Atlantic Bay scallop ( Argopecten irradians)".AQUACULTURE REPORTS 39(2024):10.

入库方式： OAI收割

来源：烟台海岸带研究所

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