Surface Blocking-Based Potentiometric Biosensor for Detection of E. coli ATCC 15597 Using Phage MS2 as a Receptor
文献类型：期刊论文
| 作者 | Wang, Kaikai1,2,4; Liang, Rongning1,2 ; Qin, Wei1,2,3,5
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| 刊名 | ACS SENSORS
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| 出版日期 | 2024-10-23 |
| 页码 | 10 |
| 关键词 | polymeric membrane ion-selective electrode surfaceblocking effect phage delayed Nernstian response bacterial detection |
| ISSN号 | 2379-3694 |
| DOI | 10.1021/acssensors.4c02004 |
| 通讯作者 | Liang, Rongning(rnliang@yic.ac.cn) ; Qin, Wei(wqin@yic.ac.cn) |
| 英文摘要 | Nowadays, using a potentiometric ion sensor to achieve detection of biological analytes is still a big challenge, since these analytes usually do not yield a measurable ion signal. To address this challenge, a simple and robust potentiometric sensing protocol based on a delayed Nernstian response is proposed for the label-free detection of biological analytes. The proposed sensor platform is composed of two layers: the surface recognition layer and the indicator-ion-selective electrode (ISE) membrane layer. It is based on a surface blocking mechanism in which the surface recognition interactions between the surface recognition element and the target can impede the diffusion of the indicator ion from the aqueous phase to the sensing membrane phase to reach the final Nernstian-response equilibrium, thus resulting in a delayed potential change. Such a potential change could be used to measure the concentration of a biological target in the sample. Thus, a sensing system was designed by using phage MS2, its host bacterium Escherichia coli ATCC 15597(abbreviated as E. coli H), and a solid-contact butyrylcholine ISE as a surface recognition element, a target, and an indicator electrode, respectively. This new concept offers a simple, sensitive, and extremely selective potentiometric method for detection of E. coli H with a detection limit of 100 CFU mL-1. It can be expected that the present approach may pave the way to using ISEs to detect various important nonionic biological targets in clinical and environmental applications. |
| WOS关键词 | ION-SELECTIVE ELECTRODES ; DETECTION LIMIT ; MEMBRANE ; POLYMER ; SENSORS ; MODULATION |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:001340137000001 |
| 资助机构 | Key Technology Research and Development Program of Shandong Province ; National Natural Science Foundation of China ; Shandong Provincial Natural Science Foundation ; Key Research Development Program of Shandong Province ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences ; Taishan Scholar Program of Shandong Province ; Special Supporting Project of Yantai (the Special Fund for the Scholar Program of Yantai) |
| 源URL | [http://ir.yic.ac.cn/handle/133337/37806]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 烟台海岸带研究所_近岸生态与环境实验室 |
| 通讯作者 | Liang, Rongning; Qin, Wei |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Beijing, Peoples R China 2.YICCAS, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Kaikai,Liang, Rongning,Qin, Wei. Surface Blocking-Based Potentiometric Biosensor for Detection of E. coli ATCC 15597 Using Phage MS2 as a Receptor[J]. ACS SENSORS,2024:10. |
| APA | Wang, Kaikai,Liang, Rongning,&Qin, Wei.(2024).Surface Blocking-Based Potentiometric Biosensor for Detection of E. coli ATCC 15597 Using Phage MS2 as a Receptor.ACS SENSORS,10. |
| MLA | Wang, Kaikai,et al."Surface Blocking-Based Potentiometric Biosensor for Detection of E. coli ATCC 15597 Using Phage MS2 as a Receptor".ACS SENSORS (2024):10. |
入库方式： OAI收割
来源：烟台海岸带研究所
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