Phylogenetic distribution and characterization of conserved C-di-GMP metabolizing proteins in filamentous cyanobacterium Arthrospira
文献类型：期刊论文
| 作者 | Wang, Kang1,2; Li, Wenjun1 ; Cui, Hongli1; Qin, Song1
|
| 刊名 | GENE
 |
| 出版日期 | 2024-11-15 |
| 卷号 | 927页码:11 |
| 关键词 | Arthrospira c-di-GMP Diguanylate cyclase Phosphodiesterase Extracellular polymeric substances |
| ISSN号 | 0378-1119 |
| DOI | 10.1016/j.gene.2024.148643 |
| 通讯作者 | Wang, Kang(wangkang211@mails.ucas.ac.cn) ; Qin, Song(sqin@yic.ac.cn) |
| 英文摘要 | Cyclic diguanosine monophosphate (c-di-GMP) is a second messenger in bacteria that regulates multiple biological functions, including biofilm formation, virulence, and intercellular communication. However, c-di-GMP signaling is virtually unknown in economically important filamentous cyanobacteria, Arthrospira. In this study, we predicted 31 genes encoding GGDEF-domain proteins from A. platensis NIES39 as potential diguanylate cyclases (DGCs). Phylogenetic distribution analysis showed five genes (RS09460, RS04865, RS26155, M01840, and E02220) with highly conserved distribution across 25 Arthrospira strains. Adc1 encoded by RS09460 was further characterized as a typical DGC. By establishing the genetic transformation system of Arthrospira, we demonstrated that the overexpression of Adc1 promoted the production of extracellular polymeric substances (EPS), which in turn caused the aggregation of filaments. We also confirmed that RS04865 and RS26155 may encode active DGCs, while enzymatic activity assays showed that proteins encoded by M01840 and E02220 have phosphodiesterase (PDE) activity. Meta-analysis revealed that the expression profiles of RS09460 and RS04865 were unaffected under 31 conditions, suggesting that they may function as conserved genes in maintaining the basal level of c-di-GMP in Arthrospira. In summary, this report will provide the basis for further studies of c-diGMP signal in Arthrospira. |
| WOS关键词 | VIBRIO-CHOLERAE ; TRANSFORMATION |
| WOS研究方向 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:001345612500001 |
| 资助机构 | Natural Science Foundation of Shandong Province ; Major Science and Technology Innovation Project of Shandong Province ; High-tech industrialization special project in cooperation between Jilin Province ; Chinese Academy of Sciences ; National Natural Science Foundation of China |
| 源URL | [http://ir.yic.ac.cn/handle/133337/37838]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 通讯作者 | Wang, Kang; Qin, Song |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Kang,Li, Wenjun,Cui, Hongli,et al. Phylogenetic distribution and characterization of conserved C-di-GMP metabolizing proteins in filamentous cyanobacterium Arthrospira[J]. GENE,2024,927:11. |
| APA | Wang, Kang,Li, Wenjun,Cui, Hongli,&Qin, Song.(2024).Phylogenetic distribution and characterization of conserved C-di-GMP metabolizing proteins in filamentous cyanobacterium Arthrospira.GENE,927,11. |
| MLA | Wang, Kang,et al."Phylogenetic distribution and characterization of conserved C-di-GMP metabolizing proteins in filamentous cyanobacterium Arthrospira".GENE 927(2024):11. |
入库方式： OAI收割
来源：烟台海岸带研究所
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