iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid
文献类型：期刊论文
| 作者 | Wang, Xin1,2,3; Wang, Dan1,2,3; Zhang, Tianyu1,2,3; Zhang, Qianqian1,2,4 ; Zhao, Jianmin1,2,4
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| 刊名 | FRONTIERS IN MARINE SCIENCE
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| 出版日期 | 2021-12-08 |
| 卷号 | 8页码:12 |
| 关键词 | okadaic acid Chlamys farreri oxidative stress detoxification genes iTRAQ |
| DOI | 10.3389/fmars.2021.792050 |
| 通讯作者 | Zhang, Qianqian(qqzhang@yic.ac.cn) ; Zhao, Jianmin(jmzhao@yic.ac.cn) |
| 英文摘要 | Okadaic acid (OA), produced by dinoflagellates during harmful algal blooms, is a principal diarrhetic shellfish poisoning toxin. This toxin poses a potential threat to bivalves with economic values. To better understand the toxicity mechanism of OA to bivalves, in this study, oxidative stress biomarkers (superoxide dismutase, SOD; catalase, CAT; glutathione S-transferase, GST; malondialdehyde, MDA) and the expression of detoxification genes (heat shock protein 70, HSP70; heat shock protein 90, HSP90; cytochrome P450, CYP450) were assessed in the gills of scallops Chlamys farreri after 24 h, 48 h and 96 h exposure to OA. In addition, the digestive glands of scallops exposed to OA for 96 h were dissected for an iTRAQ based quantitative proteomic analysis. The results of OA exposure experiments showed that OA induces oxidative stress and significant enhancement of the expression of detoxification genes in scallops. The proteomics analysis revealed that 159 proteins altered remarkably in OA-treated scallops, and these proteins were involved in phagosomes, regulation of actin cytoskeleton, adherens junction, tight junction, and focal adhesion. Amino acid biosynthesis, carbon metabolism, pentose phosphate pathway, fructose and mannose metabolism in the digestive glands were also significantly impacted. Our data shed new insights on the molecular responses and toxicity mechanisms of C. farreri to OA. |
| WOS关键词 | MYTILUS-GALLOPROVINCIALIS ; OXIDATIVE STRESS ; GENE-EXPRESSION ; ALGAL BLOOMS ; SUPEROXIDE-DISMUTASE ; ANTIOXIDANT ENZYMES ; PROROCENTRUM-LIMA ; MARINE TOXINS ; UNIO-TUMIDUS ; F-ACTIN |
| WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000732816700001 |
| 资助机构 | Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key Research and Development Program of China ; Youth Innovation Promotion Association, Chinese Academy of Sciences |
| 源URL | [http://ir.yic.ac.cn/handle/133337/37944]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 海岸带生物资源高效利用研究与发展中心 |
| 通讯作者 | Zhang, Qianqian; Zhao, Jianmin |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai, Peoples R China 3.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xin,Wang, Dan,Zhang, Tianyu,et al. iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid[J]. FRONTIERS IN MARINE SCIENCE,2021,8:12. |
| APA | Wang, Xin,Wang, Dan,Zhang, Tianyu,Zhang, Qianqian,&Zhao, Jianmin.(2021).iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid.FRONTIERS IN MARINE SCIENCE,8,12. |
| MLA | Wang, Xin,et al."iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid".FRONTIERS IN MARINE SCIENCE 8(2021):12. |
入库方式： OAI收割
来源：烟台海岸带研究所
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