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Molecular and functional characterization of short peptidoglycan recognition proteins in Vesicomyidae clam

文献类型：期刊论文

作者Kong, Xue2,3,4,5; Wang, Wei4,5; Xia, Sunan5; Zhi, Ying5; Cai, Yuefeng3,4,5; Zhang, Haibin1; Shen, Xin3,4,5
刊名DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
出版日期2025
卷号162页码:16
关键词Vesicomyidae clam Peptidoglycan recognition protein Symbiosis Cold seep zone
ISSN号0145-305X
DOI10.1016/j.dci.2024.105284
英文摘要Within cold seep environments, the Vesicomyidae clam emerges as a prevalent species, distinguished by its symbiotic relationship with microorganisms housed within its organ gill. Given the extreme conditions and the symbiotic nature of this association, investigating the host's immune genes, particularly immune recognition receptors, is essential for understanding their role in facilitating host-symbiotic interactions. Three short peptidoglycan recognition proteins (PGRPs) were identified in the clam. AmPGRP-S1, -S2, and -S3 were found to possess conserved amidase binding sites and Zn2+ binding sites. Quantitative Real-time PCR (qRT-PCR) analysis revealed differential expression patterns among the PGRPs. AmPGRP-S1 and AmPGRP-S2 exhibited elevated expression levels in the gill, while AmPGRP-S3 displayed the highest expression in the adductor muscle. Functional experiments demonstrated that recombinant AmPGRP-S1, -S2, and -S3 (rAmPGRPs) exhibited binding capabilities to both L-PGN and D-PGN (peptidoglycan). Notably, rAmPGRP-S1 and -S2 possessed Zn2+-independent amidase activity, while rAmPGRP-S3 lacked this enzymatic function. rAmPGRPs were shown to bind to five different bacterial species. Among these, rAmPGRP-S1 inhibited Escherichia coli and Bacillus subtilis, while rAmPGRP-S2 and -S3 inhibited Bacillus subtilis in the absence of Zn2+. In the presence of Zn2+, rAmPGRP-S1 and -S2 exhibited enhanced inhibitory activity against Staphylococcus aureus or Bacillus subtilis. These findings suggest that AmPGRPs may play a pivotal role in mediating the interaction between the host and endosymbiotic bacteria, functioning as PGN and microbe receptors, antibacterial effectors, and regulators of host-microbe symbiosis. These results contribute to our understanding of the adaptive mechanisms of deep-sea organisms to the challenging cold seep environments.
WOS关键词MUSSEL BATHYMODIOLUS-AZORICUS ; IMMUNE RECOGNITION ; WATER MUSSEL ; GENE ; EXPRESSION ; IDENTIFICATION ; DIVERSITY ; CLONING ; INVOLVEMENT ; RECEPTORS
资助项目Natural Science Foundation of Jiangsu Province, China[BK20210927] ; National Nature Science Foundation of China[42376139] ; Open Project of Key Laboratory of Marine Biogenetic Resources, Ministry of Natural Resources, China[HY202401] ; Jiangsu Agriculture Science and Technology Innovation Fund (JASTIF) , China[CX (22) 2032] ; Strategic Priority Research Program of the Chinese Academy of Sciences (CAS) , China[XDA22050303]
WOS研究方向Fisheries ; Immunology ; Veterinary Sciences ; Zoology
语种英语
WOS记录号WOS:001351614900001
出版者ELSEVIER SCI LTD
资助机构Natural Science Foundation of Jiangsu Province, China ; National Nature Science Foundation of China ; Open Project of Key Laboratory of Marine Biogenetic Resources, Ministry of Natural Resources, China ; Jiangsu Agriculture Science and Technology Innovation Fund (JASTIF) , China ; Strategic Priority Research Program of the Chinese Academy of Sciences (CAS) , China
源URL[http://ir.idsse.ac.cn/handle/183446/11812]  
专题深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
通讯作者Shen, Xin
作者单位1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
2.Minist Nat Resources, Inst Oceanog 3, Key Lab Marine Biogenet Resources, Xiamen 361000, Peoples R China
3.Jiangsu Ocean Univ, Coinnovat Ctr Jiangsu Marine Bioind Technol, Lianyungang 222000, Peoples R China
4.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Biotechnol, Jiangsu Key Lab Marine Bioresources & Environm, Lianyungang 222000, Peoples R China
5.Jiangsu Ocean Univ, Sch Marine Sci & Fisheries, Lianyungang 222000, Peoples R China
推荐引用方式
GB/T 7714		 Kong, Xue,Wang, Wei,Xia, Sunan,et al. Molecular and functional characterization of short peptidoglycan recognition proteins in Vesicomyidae clam[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2025,162:16.
APA Kong, Xue.,Wang, Wei.,Xia, Sunan.,Zhi, Ying.,Cai, Yuefeng.,...&Shen, Xin.(2025).Molecular and functional characterization of short peptidoglycan recognition proteins in Vesicomyidae clam.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,162,16.
MLA Kong, Xue,et al."Molecular and functional characterization of short peptidoglycan recognition proteins in Vesicomyidae clam".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 162(2025):16.

入库方式： OAI收割

来源：深海科学与工程研究所

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