Developing a Flow-Resistance Module for Elucidating Cell Mechanotransduction on Multiple Shear Stresses
文献类型：期刊论文
| 作者 | Zhang ZL(张子良)2,3,4,5; Zheng Z(郑植)1,2,3; Gao YX(高宇欣)2,3 ; Li W(李旺)1,2,3; Zhang XY(张晓宇)1,2,3; Luo H(罗欢)2,3; Lv SQ(吕守芹)1,2,3; Du Y(杜宇)2,3; Zhang Y(章燕)1,2,3; Li N(李宁)1,2,3
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| 刊名 | ACS BIOMATERIALS SCIENCE & ENGINEERING
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| 出版日期 | 2024-12-16 |
| 页码 | 13 |
| 关键词 | flow resistance microfluidic platform endothelialcells HCC cells mechanobiology |
| ISSN号 | 2373-9878 |
| DOI | 10.1021/acsbiomaterials.4c01604 |
| 通讯作者 | Li, Ning(lining_1@imech.ac.cn) ; Long, Mian(mlong@imech.ac.cn) |
| 英文摘要 | Fluid shear stress plays a pivotal role in regulating cellular behaviors, maintaining tissue homeostasis, and driving disease progression. Cells in various tissues are specifically adapted to physiological levels of shear stress and exhibit sensitivity to variations in its magnitude, highlighting the requirement for a comprehensive understanding of cellular responses to both physiologically and pathologically relevant levels of shear stress. In this study, we developed an independent upstream flow-resistance module with high fluidic resistances comprising three microchannels. The validity of the flow-resistance module was confirmed via computational fluid dynamics (CFD) simulations and flow calibration experiments, resulting in the generation of steady wall shear stresses ranging from 0.06 to 11.57 dyn/cm2 within the interconnected cell culture chips. Gene expression profiles, cytoskeletal remodeling, and morphological changes, as well as Yes-associated protein (YAP) nuclear translocation, were investigated in response to various shear stresses to authenticate the reliability of our experimental platform, indicating an increasing trend as the shear stress increases, reaching its maximum at various shear stresses. Our findings suggest that this flow-resistance module can be readily employed for precise characterization of cellular responses under various shear stresses. |
| 分类号 | 二类 |
| WOS关键词 | ENDOTHELIAL-CELLS ; NEUTROPHILS ; EXPOSURE ; PLATFORM ; ALPHA |
| 资助项目 | National Natural Science Foundation of China[32130061] ; National Natural Science Foundation of China[T2394512] ; National Natural Science Foundation of China[32250017] ; National Natural Science Foundation of China[32271366] ; China Manned Space Flight Technology Project ; Chinese Space Station Experiment Project[YYW-T0901EXP0701] ; Key Research Program of Chinese Academy of Sciences[ZDBS-ZRKJZ-TLC002] |
| WOS研究方向 | Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001378697200001 |
| 资助机构 | National Natural Science Foundation of China ; China Manned Space Flight Technology Project ; Chinese Space Station Experiment Project ; Key Research Program of Chinese Academy of Sciences |
| 其他责任者 | Li, Ning ; Long, Mian |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/98009]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China; 3.Chinese Acad Sci, Ctr Biomech & Bioengn, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China; 4.Shandong Acad Med Sci, Jinan 250117, Peoples R China; 5.Shandong First Med Univ, Med Sci & Technol Innovat Ctr, Jinan 250117, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhang ZL,Zheng Z,Gao YX,et al. Developing a Flow-Resistance Module for Elucidating Cell Mechanotransduction on Multiple Shear Stresses[J]. ACS BIOMATERIALS SCIENCE & ENGINEERING,2024:13. |
| APA | 张子良.,郑植.,高宇欣.,李旺.,张晓宇.,...&龙勉.(2024).Developing a Flow-Resistance Module for Elucidating Cell Mechanotransduction on Multiple Shear Stresses.ACS BIOMATERIALS SCIENCE & ENGINEERING,13. |
| MLA | 张子良,et al."Developing a Flow-Resistance Module for Elucidating Cell Mechanotransduction on Multiple Shear Stresses".ACS BIOMATERIALS SCIENCE & ENGINEERING (2024):13. |
入库方式： OAI收割
来源：力学研究所
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