Spreading area and shape regulate apoptosis and differentiation of osteoblasts
文献类型：期刊论文
| 作者 | Fu RR(付瑞荣) ; Liu QL(刘琴丽); Song GB; Baik A; Hu M(胡漫); Sun SJ(孙树津); Guo XE; Long M(龙勉); Huo B(霍波)
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| 刊名 | BIOMEDICAL MATERIALS
 |
| 出版日期 | 2013-10 |
| 卷号 | 8期号:5页码:055005/1-055005/8 |
| 通讯作者邮箱 | mlong@imech.ac.cn; huobo@bit.edu.cn |
| ISSN号 | 1748-6041 |
| 产权排序 | [Fu, Ruirong; Liu, Qinli; Hu, Man; Sun, Shujin; Long, Mian; Huo, Bo] Chinese Acad Sci, Key Lab Micrograv, Inst Mech, Beijing 100190, Peoples R China; [Fu, Ruirong; Liu, Qinli; Hu, Man; Sun, Shujin; Long, Mian; Huo, Bo] Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China; [Liu, Qinli; Song, Guanbin] Chongqing Univ, Coll Bioengn, Chongqing 400030, Peoples R China; [Baik, Andrew; Guo, X. Edward] Columbia Univ, Dept Biomed Engn, New York, NY USA; [Huo, Bo] Beijing Inst Technol, Sch Aerosp Engn, Beijing 100081, Peoples R China |
| 通讯作者 | Long, M (reprint author), Chinese Acad Sci, Key Lab Micrograv, Inst Mech, Beijing 100190, Peoples R China. |
| 合作状况 | 国际 |
| 中文摘要 | The in vivo observations have indicated that at the remodeling sites of bone, the spreading area or shape of preosteoblasts is confined by the mineralized matrix. But it remains unknown whether this spreading confinement regulates the differentiation or apoptosis of osteoblasts. In the present study, osteoblast-like cells (MC3T3-E1) were seeded on micropatterned islands with different area and shape. The expression of three osteogenic differentiation markers was measured by immunofluorescence staining and apoptotic cells were detected using a terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labelling assay kit. The membrane fluorescence staining results showed that the actual spreading area of micropatterned osteoblasts coincided with the designed value. When the area of a micropatterned cell was confined as 314 or 615 mu m(2), which was lower than that of freely spreading osteoblasts, the circular shape promoted the expression of osteogenic differentiation markers and the percentage of apoptotic osteoblasts compared with the branched shape. This shape-regulated differentiation and apoptosis of osteoblasts with confined spreading area were abolished when actin polymerization was inhibited by cytochalasin D. The present study gives an insight into the roles of spreading morphology on osteoblastic differentiation and apoptosis. |
| 学科主题 | 生物力学 |
| 分类号 | 二类 |
| 收录类别 | SCI ; EI |
| 资助信息 | National Natural Science Foundation of China [30970707, 31110103918]; National Key Basic Research Foundation of China [2011CB710904] |
| 原文出处 | http://dx.doi.org/10.1088/1748-6041/8/5/055005 |
| 语种 | 英语 |
| WOS记录号 | WOS:000324922200005 |
| 公开日期 | 2013-10-28 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/47499]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Fu RR,Liu QL,Song GB,et al. Spreading area and shape regulate apoptosis and differentiation of osteoblasts[J]. BIOMEDICAL MATERIALS,2013,8(5):055005/1-055005/8. |
| APA | 付瑞荣.,刘琴丽.,Song GB.,Baik A.,胡漫.,...&霍波.(2013).Spreading area and shape regulate apoptosis and differentiation of osteoblasts.BIOMEDICAL MATERIALS,8(5),055005/1-055005/8. |
| MLA | 付瑞荣,et al."Spreading area and shape regulate apoptosis and differentiation of osteoblasts".BIOMEDICAL MATERIALS 8.5(2013):055005/1-055005/8. |
入库方式： OAI收割
来源：力学研究所
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