Development of CadR-based cadmium whole cell biosensor for visual detection of environmental Cd2+
文献类型：期刊论文
| 作者 | Zhang, Tianyi1; Zhu, KaiLi3; Zhang, Xia1; Yu, Xin1; Shen, Liang1; Gao, Defeng1; Chen, Yiwen1; Wang, Qinghua1; Chen, Shaopeng1 ; Bao, Lingzhi1,2
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| 刊名 | ANALYTICA CHIMICA ACTA
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| 出版日期 | 2024-11-22 |
| 卷号 | 1330 |
| 关键词 | Whole-cell biosensor Cadmium Constitutive promoter Visualization 5 '-UTRs |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2024.343299 |
| 通讯作者 | Chen, Shaopeng(20200080@wnmc.edu.cn) ; Bao, Lingzhi(20200002@wnmc.edu.cn) |
| 英文摘要 | Background: As a threat to human health and public health, cadmium (Cd) pollution has received widespread social concern. Our previously constructed CadR-based bacterial whole cell biosensor (WCB) epCadR5 showed high sensitivity and specificity in cadmium detection. However, the application of the sensor is still hindered by the need for laboratory equipment to read the fluorescence signal output. In this study, we aimed to optimizing the sensor to make it available for visual detection of environmental cadmium and simplify the detection process to advance practical application of the sensor. Results: By replacing the constitutive promoter with J110, the fluorescence signal output of the sensor was significantly increased and the fluorescence leakage was decreased. In addition, the fluorescence signal output of green fluorescence protein (GFP) was enhanced by the addition of a 5 ' untranslated region (5 '-UTR) mlcR10. The fluorescence signal output of the WCB is sufficiently robust to be visible and distinguishable to the naked eye, which is of paramount importance for visual detection. The sensor readout can be conveniently recorded by mobile phone camera and quantified. For ease of on-site application, the WCB's visual detection procedures and conditions were further optimized and simplified. The WCB demonstrated good linearity and detection limit (1.81 mu g/L) for visual detection of Cd2+ without the assistance of bulky laboratory equipment. For the detection of real environmental samples, the WCB visual detection results were close to those of WCB-flow cytometry (FACS) and graphite furnace atomic absorption spectroscopy (GFAAS). Significance: In this work, we developed an easy-to-use, on-site and visual detection biosensor for monitoring environmental Cd2+. It will advance the utilization of cadmium WCBs in practical settings. The optimization and simplification strategy in the study also provide new insights into the visualization of other bacterial biosensors, and will advance the practical application of WCBs. |
| WOS关键词 | ESCHERICHIA-COLI ; CIRCUITS ; PROTEIN |
| 资助项目 | Open Foundation of Information Materials and Intelligent Sensing Laboratory of Anhui Province, Anhui University[IMIS202202] ; Natural Science Research Project of Education Department of Anhui Province[2023AH040241] ; Anhui Provincial Key R D Programs[202004i07020016] ; National College Students Innovation and Entrepreneurship Training Program[202210368007] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001332999400001 |
| 出版者 | ELSEVIER |
| 资助机构 | Open Foundation of Information Materials and Intelligent Sensing Laboratory of Anhui Province, Anhui University ; Natural Science Research Project of Education Department of Anhui Province ; Anhui Provincial Key R D Programs ; National College Students Innovation and Entrepreneurship Training Program |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/134642]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Chen, Shaopeng; Bao, Lingzhi |
| 作者单位 | 1.Wannan Med Coll, Sch Publ Hlth, Wuhu 241002, Anhui, Peoples R China 2.Anhui Univ, Informat Mat & Intelligent Sensing Lab Anhui Prov, Hefei 230039, Anhui, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Environm Toxicol & Pollut Control Technol, Hefei 230031, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Tianyi,Zhu, KaiLi,Zhang, Xia,et al. Development of CadR-based cadmium whole cell biosensor for visual detection of environmental Cd2+[J]. ANALYTICA CHIMICA ACTA,2024,1330. |
| APA | Zhang, Tianyi.,Zhu, KaiLi.,Zhang, Xia.,Yu, Xin.,Shen, Liang.,...&Bao, Lingzhi.(2024).Development of CadR-based cadmium whole cell biosensor for visual detection of environmental Cd2+.ANALYTICA CHIMICA ACTA,1330. |
| MLA | Zhang, Tianyi,et al."Development of CadR-based cadmium whole cell biosensor for visual detection of environmental Cd2+".ANALYTICA CHIMICA ACTA 1330(2024). |
入库方式： OAI收割
来源：合肥物质科学研究院
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