Enhanced molecular stability of ApxII antigen during secretion in Corynebacterium glutamicum by rational design
文献类型：期刊论文
| 作者 | Liu, Xiuxia2,3,4; Yang, Shujie2,3,4; Sun, Manman1; Gao, Alex Xiong5; Fan, Ziming2,3,4; Yang, Yankun2,3,4; Zheng, Pei6; Liu, Chunli2,3,4; Li, Ye2,3,4; Bai, Zhonghu2,3,4 |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2024-11-10 |
| 卷号 | 394 |
| 关键词 | Truncated ApxII Molecular stability towards proteolytic degradation Corynebacterium glutamicum Rational design |
| ISSN号 | 0168-1656 |
| DOI | 10.1016/j.jbiotec.2024.08.003 |
| 通讯作者 | Yang, Yankun(yangyankun@jiangnan.edu.cn) |
| 英文摘要 | ApxII is a vaccine antigen used to protect against porcine contagious pleuropneumonia, which is a significant threat to the pig industry. Here, we aimed to improve the proteolytic degradation stability of ApxII during its secretion by establishing a complete screening process of stable variants through bioinformatics and site-directed mutagenesis. We employed a combination of semi-rational and rational design strategies to create 34 single-point variants of ApxII. Among them, R114E and T115D variants exhibited better stability without compromising antigen activity. Furthermore, we constructed a multi-site variant, R114E/T115D, which demonstrated the best stability, activity, and yield. Protein stability and molecular dynamic analysis indicated that the greater solubility and lower structural expansion coefficient might explain the increased stability of R114E/T115D. Additionally, site T115 was identified as a key point of truncated ApxII stability. The R114E/T115D variant, with its proven stability and intact antigenic activity, holds promising prospects for industrial-scale applications in the prevention of porcine contagious pleuropneumonia. |
| WOS关键词 | ACTINOBACILLUS-PLEUROPNEUMONIAE ; RESISTANCE ; CHALLENGE ; VACCINE |
| 资助项目 | National Key Research and Devel-opment Program of China[2023YFC3402400] ; National Natural Science Foundation of China[22378167] ; National Natural Science Foundation of China[22078128] ; National Natural Science Foundation of China[21938004] ; Fundamental Research Funds for the Central Universities[JUSRP221032] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001301368100001 |
| 出版者 | ELSEVIER |
| 资助机构 | National Key Research and Devel-opment Program of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/134868]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Yang, Yankun |
| 作者单位 | 1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei, Peoples R China 2.Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Peoples R China 3.Jiangnan Univ, Natl Engn Res Ctr Cereal Fermentat & Food Biomfg, Wuxi 214112, Peoples R China 4.Jiangnan Univ, Jiangsu Prov Res Ctr Bioact Prod Proc Technol, Wuxi 214122, Peoples R China 5.Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Peoples R China 6.Tecon Biol CO Ltd, Urumqi 83000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xiuxia,Yang, Shujie,Sun, Manman,et al. Enhanced molecular stability of ApxII antigen during secretion in Corynebacterium glutamicum by rational design[J]. JOURNAL OF BIOTECHNOLOGY,2024,394. |
| APA | Liu, Xiuxia.,Yang, Shujie.,Sun, Manman.,Gao, Alex Xiong.,Fan, Ziming.,...&Bai, Zhonghu.(2024).Enhanced molecular stability of ApxII antigen during secretion in Corynebacterium glutamicum by rational design.JOURNAL OF BIOTECHNOLOGY,394. |
| MLA | Liu, Xiuxia,et al."Enhanced molecular stability of ApxII antigen during secretion in Corynebacterium glutamicum by rational design".JOURNAL OF BIOTECHNOLOGY 394(2024). |
入库方式： OAI收割
来源：合肥物质科学研究院
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