Activin A plays an essential role in migration and proliferation of hepatic stellate cells via Smad3 and calcium signaling
文献类型：期刊论文
| 作者 | Zhang, Wei2; Zhu, Linjing1; Fang, Fang1; Zhang, Fenglin3; Wang, Runnan1; Yang, Ke4 ; Liu, Yahui2; Cui, Xueling1
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| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2024-09-03 |
| 卷号 | 14 |
| 关键词 | Hepatic stellate cells Proliferation Migration Activin A Smad3 Calcium signaling |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-024-71304-7 |
| 通讯作者 | Liu, Yahui(yahui@jlu.edu.cn) ; Cui, Xueling(cxl@jlu.edu.cn) |
| 英文摘要 | Activin A and hepatic stellate cells (HSCs) are involved in tissue repair and fibrosis in liver injury. This study investigated the impact of activin A on HSC activation and migration. A microfluidic D4-chip was used for examining the cell migration of mouse hepatic stellate cell line MHSteC. The analysis of differentially expressed genes revealed that activin beta A (Inhba), activin receptor type 1A (Acvr1a) and type 2A (Acvr2a) mRNAs were more significantly expressed in human HSCs than in the hepatocytes. Moreover, activin A promoted MHSteC proliferation and induced MHSteC migration. Furthermore, the MHSteCs treated with activin A exhibited increased levels of migration-related proteins, N-cadherin, Vimentin, alpha-SMA, MMP2 and MMP9, but a decreased level of E-cadherin. Additionally, activin A treatment significantly increased the p-Smad3 levels and p-Smad3/Smad3 ratio in the MHSteCs, and the Smad3 inhibitor SIS3 attenuated activin A-induced MHSteC proliferation and migration. Simultaneously, activin A increased the calcium levels in the MHSteCs, and the migratory effects of activin A on MHSteCs were weakened by the intracellular calcium ion-chelating agent BAPTA-AM. These data indicate that activin A can promote MHSteC activation and migration through the canonical Smad3 signaling and calcium signaling. |
| WOS关键词 | EXPRESSION ; FOLLISTATIN ; INFLAMMATION ; ACTIVATION ; INDUCTION ; FIBROSIS |
| 资助项目 | Foundation of Science and Technology Department of Jilin Province ; [20220402079GH] ; [20240404027ZP] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:001304249300003 |
| 出版者 | NATURE PORTFOLIO |
| 资助机构 | Foundation of Science and Technology Department of Jilin Province |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/134975]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Liu, Yahui; Cui, Xueling |
| 作者单位 | 1.Jilin Univ, Coll Basic Med Sci, Dept Genet, Changchun 130021, Jilin, Peoples R China 2.First Hosp Jilin Univ, Gen Surg Ctr, Dept Hepatobiliary & Pancreat Surg, Changchun 130021, Jilin, Peoples R China 3.Jilin Univ, Coll Basic Med Sci, Dept Immunol, Changchun 130021, Jilin, Peoples R China 4.Chinese Acad Sci, Inst Appl Technol, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Wei,Zhu, Linjing,Fang, Fang,et al. Activin A plays an essential role in migration and proliferation of hepatic stellate cells via Smad3 and calcium signaling[J]. SCIENTIFIC REPORTS,2024,14. |
| APA | Zhang, Wei.,Zhu, Linjing.,Fang, Fang.,Zhang, Fenglin.,Wang, Runnan.,...&Cui, Xueling.(2024).Activin A plays an essential role in migration and proliferation of hepatic stellate cells via Smad3 and calcium signaling.SCIENTIFIC REPORTS,14. |
| MLA | Zhang, Wei,et al."Activin A plays an essential role in migration and proliferation of hepatic stellate cells via Smad3 and calcium signaling".SCIENTIFIC REPORTS 14(2024). |
入库方式： OAI收割
来源：合肥物质科学研究院
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